Requirement for the yeast gene LON in intramitochondrial proteolysis and maintenance of respiration

Carolyn K. Suzuki, Kitaru Suda, Nan Wang, Gottfried Schatz

Research output: Contribution to journalArticlepeer-review

183 Scopus citations

Abstract

The role of protein degradation in mitochondrial homeostasis was explored by cloning of a gene from Saccharomyces cerevisiae that encodes a protein resembling the adenosine triphosphate (ATP)-dependent bacterial protease Lon. The predicted yeast protein has a typical mitochondrial matrix-targeting sequence at its amino terminus. Yeast cells lacking a functional LON gene contained a nonfunctional mitochondrial genome, were respiratory-deficient, and lacked an ATP-dependent proteolytic activity present in the mitochondria of Lon+ cells. Lon- cells were also impaired in their ability to catalyze the energy-dependent degradation of several mitochondrial matrix proteins and they accumulated electron-dense inclusions in their mitochondrial matrix.

Original languageEnglish (US)
Pages (from-to)273-276
Number of pages4
JournalScience
Volume264
Issue number5156
DOIs
StatePublished - 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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