Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17

Ki Young Choi; Dockyu Kim; Jong Chan Chae; Gerben J. Zylstra; Eungbin Kim

doi:10.1016/j.bbrc.2007.04.009

Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17

Ki Young Choi, Dockyu Kim, Jong Chan Chae, Gerben J. Zylstra, Eungbin Kim

School of Environmental and Biological Sciences, Biochemistry & Microbiology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The operons encoding the transformation of phthalate to protocatechuate are duplicated and present on two different megaplasmids [pDK2 (330 kb) and pDK3 (750 kb)] in Rhodococcus sp. strain DK17. RT-PCR experiments using gene-specific primers showed that both the pDK2- and the pDK3-encoded dihydroxyphthalate decarboxylase genes are simultaneously expressed during growth on phthalate. The doubling time of the pDK2-cured mutant strain DK176 in minimal liquid medium with 5 mM phthalate is 52.5% of that of the wild-type strain DK17. The data indicate that both copies of the phthalate operon are equally functional in DK17, and gene dosage is the main reason for slower growth of DK176 on phthalate.

Original language	English (US)
Pages (from-to)	766-771
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	357
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2007.04.009
State	Published - Jun 8 2007

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Keywords

Gene duplication
Megaplasmid
Operon
Phthalate
Rhodococcus

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10.1016/j.bbrc.2007.04.009

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title = "Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17",

abstract = "The operons encoding the transformation of phthalate to protocatechuate are duplicated and present on two different megaplasmids [pDK2 (330 kb) and pDK3 (750 kb)] in Rhodococcus sp. strain DK17. RT-PCR experiments using gene-specific primers showed that both the pDK2- and the pDK3-encoded dihydroxyphthalate decarboxylase genes are simultaneously expressed during growth on phthalate. The doubling time of the pDK2-cured mutant strain DK176 in minimal liquid medium with 5 mM phthalate is 52.5% of that of the wild-type strain DK17. The data indicate that both copies of the phthalate operon are equally functional in DK17, and gene dosage is the main reason for slower growth of DK176 on phthalate.",

keywords = "Gene duplication, Megaplasmid, Operon, Phthalate, Rhodococcus",

author = "Choi, {Ki Young} and Dockyu Kim and Chae, {Jong Chan} and Zylstra, {Gerben J.} and Eungbin Kim",

note = "Funding Information: This work was supported by a grant from the Ministry of Science and Technology, Republic of Korea through the 21C Frontier Microbial Genomics and Applications Center Program and by a grant from KOSEF through AEBRC at POSTECH. K.C. is a recipient of the Brain Korea 21 scholarship. G.J.Z. acknowledges the support of NSF through grants MCB-0078465 and CHE-0221978.",

year = "2007",

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doi = "10.1016/j.bbrc.2007.04.009",

language = "English (US)",

volume = "357",

pages = "766--771",

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T1 - Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17

AU - Choi, Ki Young

AU - Kim, Dockyu

AU - Chae, Jong Chan

AU - Zylstra, Gerben J.

AU - Kim, Eungbin

N1 - Funding Information: This work was supported by a grant from the Ministry of Science and Technology, Republic of Korea through the 21C Frontier Microbial Genomics and Applications Center Program and by a grant from KOSEF through AEBRC at POSTECH. K.C. is a recipient of the Brain Korea 21 scholarship. G.J.Z. acknowledges the support of NSF through grants MCB-0078465 and CHE-0221978.

PY - 2007/6/8

Y1 - 2007/6/8

N2 - The operons encoding the transformation of phthalate to protocatechuate are duplicated and present on two different megaplasmids [pDK2 (330 kb) and pDK3 (750 kb)] in Rhodococcus sp. strain DK17. RT-PCR experiments using gene-specific primers showed that both the pDK2- and the pDK3-encoded dihydroxyphthalate decarboxylase genes are simultaneously expressed during growth on phthalate. The doubling time of the pDK2-cured mutant strain DK176 in minimal liquid medium with 5 mM phthalate is 52.5% of that of the wild-type strain DK17. The data indicate that both copies of the phthalate operon are equally functional in DK17, and gene dosage is the main reason for slower growth of DK176 on phthalate.

AB - The operons encoding the transformation of phthalate to protocatechuate are duplicated and present on two different megaplasmids [pDK2 (330 kb) and pDK3 (750 kb)] in Rhodococcus sp. strain DK17. RT-PCR experiments using gene-specific primers showed that both the pDK2- and the pDK3-encoded dihydroxyphthalate decarboxylase genes are simultaneously expressed during growth on phthalate. The doubling time of the pDK2-cured mutant strain DK176 in minimal liquid medium with 5 mM phthalate is 52.5% of that of the wild-type strain DK17. The data indicate that both copies of the phthalate operon are equally functional in DK17, and gene dosage is the main reason for slower growth of DK176 on phthalate.

KW - Gene duplication

KW - Megaplasmid

KW - Operon

KW - Phthalate

KW - Rhodococcus

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Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17

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