Restoration of embryogenic competence in repeatedly subcultured carrot cell lines

Wook Jin Chung, Henrik Pedersen, Chee Kok Chin

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


The production of somatic embryos from carrot suspension cultures invariably decreases through simple, repeated subculturing. Extracellular, concentrated compounds extracted from already established embryo culture not only recovered the embryogenic capability, but also accelerated the embryo production as much as two-fold (up to 1600 embryos/ml) compared with that of a control culture. Sugars, which were only a small portion of the total concentrate, were excluded as possible causative factors. It is likely that a protein fraction that is generated directly by competent, embryogenic cultures is important for the restoration of embryogenic potential.

Original languageEnglish (US)
Pages (from-to)545-548
Number of pages4
JournalBiotechnology Techniques
Issue number6
StatePublished - Nov 1992

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Applied Microbiology and Biotechnology


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