Restriction-modification systems and bacteriophage invasion: Who wins?

Farida N. Enikeeva, Konstantin Severinov, Mikhail S. Gelfand

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The success of a phage that infects a bacterial cell possessing a restriction-modification (R-M) system depends on the activities of the host methyltransferase and restriction endonuclease, and the number of susceptible sites in the phage genome. However, there is no model describing this dependency and linking it to observable parameters such as the fraction of surviving cells under excess phage, or probability of plating at low amount of phages. We model the phage infection of a cell with a R-M system as a pure birth process with a killing state. We calculate the transitional probabilities and the stationary distribution for this process. We generalize the model developed for a single cell to the case of multiple identical cells invaded by a Poisson-distributed number of phages. The R-M enzyme activities are assumed to be constant, time-dependent, or random. The obtained results are used to estimate the ratio of the methyltransferase and endonuclease activities from the observed fraction of surviving cells.

Original languageEnglish (US)
Pages (from-to)550-559
Number of pages10
JournalJournal of Theoretical Biology
Volume266
Issue number4
DOIs
StatePublished - Oct 1 2010

Fingerprint

DNA Restriction-Modification Enzymes
Bacteriophages
restriction endonucleases
Invasion
bacteriophages
Restriction
Cell
methyltransferases
Methyltransferases
cells
Endonucleases
DNA Restriction Enzymes
Enzyme activity
Stationary Distribution
Plating
Linking
Infection
Excess
Siméon Denis Poisson
Enzymes

All Science Journal Classification (ASJC) codes

  • Statistics and Probability
  • Modeling and Simulation
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)
  • Applied Mathematics

Keywords

  • Enzyme activities ratio
  • Methyltransferase
  • Pure birth process with killing
  • Restriction endonuclease

Cite this

Enikeeva, Farida N. ; Severinov, Konstantin ; Gelfand, Mikhail S. / Restriction-modification systems and bacteriophage invasion : Who wins?. In: Journal of Theoretical Biology. 2010 ; Vol. 266, No. 4. pp. 550-559.
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Restriction-modification systems and bacteriophage invasion : Who wins? / Enikeeva, Farida N.; Severinov, Konstantin; Gelfand, Mikhail S.

In: Journal of Theoretical Biology, Vol. 266, No. 4, 01.10.2010, p. 550-559.

Research output: Contribution to journalArticle

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