Results of a preliminary "blind" study on the presence of the human tumor nucleolar antigen in breast carcinomas, benign breast tumors, and normal breast tissues

Harris Busch, Rose K. Busch, Pui Kwong Chan, William Isenberg, Ray Weigan, Jose Russo, Philip Furmanski

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Abstract

A study was made on the presence in benign and malignant breast tumors of a common nucleolar antigen previously found in a broad range of human malignant tumors (Busch et al., Cancer Res. 39, 3024, 1979). The specimens were treated first with rabbit antibodies to "nucleolar preparations" or the nuclear 0.01 M Tris-HCl extracts of HeLa S3 cells and then with fluorescein-conjugated goat anti-rabbit antibodies. With this procedure, bright nucleolar immunofluorescence was observed in 19 20 (95%) of known breast cancer specimens. In the group of 80 unknown samples in the "blind" study, 75 (94%) were correctly identified as malignant or benign on the basis of the presence and distribution of the nucleolar fluorescence. In a group of 67 samples in which the nucleolar fluorescence was either readily observed or virtually absent, 47 48 (98%) of the malignant tumors were correctly identified. Of the benign lesions or normal breast specimens, 18 19 (95%) were correctly identified as negative for nucleolar fluorescence. These studies extend the results previously reported of a common nucleolar antigen in a broad range of human cancers to a larger series of malignancies of a particular organ. The tumor nucleolar antigen(s) were partially characterized by isoelectric focusing on 4% polyacrylamide gels. One major band had a pI of 6.3 and a minor band had a pI of 6.1. These antigens were not found in the normal human liver nucleoli.

Original languageEnglish (US)
Pages (from-to)155-167
Number of pages13
JournalClinical Immunology and Immunopathology
Volume18
Issue number2
DOIs
StatePublished - Jan 1 1981
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

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