Retention of messenger activity of RNA phage RNA following RPC-5 chromatography

Cam Campbell; Stuart M. Arfin; Emanuel Goldman

doi:10.1016/0003-2697(80)90331-0

Retention of messenger activity of RNA phage RNA following RPC-5 chromatography

Cam Campbell, Stuart M. Arfin, Emanuel Goldman

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

RNA from bacteriophages MS2 and Qβ can be chromatographed on reverse-phase-chromatography-5 (RPC-5) columns and, subsequent to the chromatography, the RNAs retain their messenger activity and direct the initiation of their respective gene products in approximately the proper relative proportions. Further, MS2 RNA chromatographs as a discrete peak even in the presence of bulk Escherichia coli RNA on these columns. Pulse-labeled E. coli RNA is spread throughout the fractions of an RPC-5 column separation, suggesting that individual bacterial messenger RNA species could be fractionated in this manner. These results indicate that RPC-5 column chromatography is a potentially useful procedure as a method for fractionating and purifying mRNA.

Original language	English (US)
Pages (from-to)	153-158
Number of pages	6
Journal	Analytical Biochemistry
Volume	102
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(80)90331-0
State	Published - Feb 1980
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(80)90331-0

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@article{4c9a65ce3e0b4085a303ee044d69dcdc,

title = "Retention of messenger activity of RNA phage RNA following RPC-5 chromatography",

abstract = "RNA from bacteriophages MS2 and Qβ can be chromatographed on reverse-phase-chromatography-5 (RPC-5) columns and, subsequent to the chromatography, the RNAs retain their messenger activity and direct the initiation of their respective gene products in approximately the proper relative proportions. Further, MS2 RNA chromatographs as a discrete peak even in the presence of bulk Escherichia coli RNA on these columns. Pulse-labeled E. coli RNA is spread throughout the fractions of an RPC-5 column separation, suggesting that individual bacterial messenger RNA species could be fractionated in this manner. These results indicate that RPC-5 column chromatography is a potentially useful procedure as a method for fractionating and purifying mRNA.",

author = "Cam Campbell and Arfin, {Stuart M.} and Emanuel Goldman",

note = "Funding Information: This work was supported in part by grants from the NIH to Stuart M. Arhn (GM 21002) and to G. Wesley Hatfield (GM 24330), and from the NSF to G. Wesley Hatfield (PCM 78-08564). Cam Campbell was a recipient of a President{\textquoteright}s Undergraduate Research Fellow Scholarship. Emanuel Goldman was a Lievre Senior Fellow (D303) of the California Division-American Cancer Society. The authors also wish to acknowledge the helpful discussions and advice contributed by Dr. G. W. Hatfield to this work.",

year = "1980",

month = feb,

doi = "10.1016/0003-2697(80)90331-0",

language = "English (US)",

volume = "102",

pages = "153--158",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Retention of messenger activity of RNA phage RNA following RPC-5 chromatography

AU - Campbell, Cam

AU - Arfin, Stuart M.

AU - Goldman, Emanuel

N1 - Funding Information: This work was supported in part by grants from the NIH to Stuart M. Arhn (GM 21002) and to G. Wesley Hatfield (GM 24330), and from the NSF to G. Wesley Hatfield (PCM 78-08564). Cam Campbell was a recipient of a President’s Undergraduate Research Fellow Scholarship. Emanuel Goldman was a Lievre Senior Fellow (D303) of the California Division-American Cancer Society. The authors also wish to acknowledge the helpful discussions and advice contributed by Dr. G. W. Hatfield to this work.

PY - 1980/2

Y1 - 1980/2

N2 - RNA from bacteriophages MS2 and Qβ can be chromatographed on reverse-phase-chromatography-5 (RPC-5) columns and, subsequent to the chromatography, the RNAs retain their messenger activity and direct the initiation of their respective gene products in approximately the proper relative proportions. Further, MS2 RNA chromatographs as a discrete peak even in the presence of bulk Escherichia coli RNA on these columns. Pulse-labeled E. coli RNA is spread throughout the fractions of an RPC-5 column separation, suggesting that individual bacterial messenger RNA species could be fractionated in this manner. These results indicate that RPC-5 column chromatography is a potentially useful procedure as a method for fractionating and purifying mRNA.

AB - RNA from bacteriophages MS2 and Qβ can be chromatographed on reverse-phase-chromatography-5 (RPC-5) columns and, subsequent to the chromatography, the RNAs retain their messenger activity and direct the initiation of their respective gene products in approximately the proper relative proportions. Further, MS2 RNA chromatographs as a discrete peak even in the presence of bulk Escherichia coli RNA on these columns. Pulse-labeled E. coli RNA is spread throughout the fractions of an RPC-5 column separation, suggesting that individual bacterial messenger RNA species could be fractionated in this manner. These results indicate that RPC-5 column chromatography is a potentially useful procedure as a method for fractionating and purifying mRNA.

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U2 - 10.1016/0003-2697(80)90331-0

DO - 10.1016/0003-2697(80)90331-0

M3 - Article

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SN - 0003-2697

VL - 102

SP - 153

EP - 158

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Retention of messenger activity of RNA phage RNA following RPC-5 chromatography

Abstract

All Science Journal Classification (ASJC) codes

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