Retinol-binding protein-deficient mice

Biochemical basis for impaired vision

Silke Vogel, Roseann Piantedosi, Sheila M. O'Byrne, Yuko Kako, Loredana Quadro, Max E. Gottesman, Ira J. Goldberg, William S. Blaner

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

We reported previously that mice lacking plasma retinol-binding protein (RBP) are phenotypically normal except that they display impaired vision at the time of weaning. This visual defect is associated with greatly diminished eyecup levels of retinaldehyde and is reversible if the mutants are maintained for several months on a vitamin A-sufficient diet. Here we provide a biochemical basis for the visual phenotype of RBP-deficient mice. This phenotype does not result from inadequate milk total retinol levels since these are not different for RBP-deficient and wild-type mice. The eye, unlike all other tissues that have been examined, takes up dietary retinol very poorly. Moreover, compared to other tissues, the eye displays a strong preference for retinol uptake when retinol is delivered bound to RBP. The poor uptake of dietary retinol by the eye coupled with its marked ability to take up retinol from RBP, we propose, provides a basis for the impaired vision observed in weanling RBP-deficient mice. Further study of the mutants suggests that the impaired vision is reversible because the eyes of mutant mice slowly acquire sufficient retinol from the low levels of retinol present in their circulation either bound to albumin or present in lipoprotein fractions. Thus, the eye is unlike other tissues in the body in that it shows a very marked preference for acquiring retinol needed to support vision from the retinol-RBP complex and is unable to meet adequately its retinol need through uptake of recently absorbed dietary retinol. This provides an explanation for the impaired vision phenotype of RBP-deficient mice.

Original languageEnglish (US)
Pages (from-to)15360-15368
Number of pages9
JournalBiochemistry
Volume41
Issue number51
DOIs
StatePublished - Dec 24 2002

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Retinol-Binding Proteins
Vitamin A
Tissue
Phenotype
Plasma Retinol-Binding Proteins
Retinaldehyde
Aptitude
Nutrition
Weaning
Lipoproteins
Albumins

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Vogel, S., Piantedosi, R., O'Byrne, S. M., Kako, Y., Quadro, L., Gottesman, M. E., ... Blaner, W. S. (2002). Retinol-binding protein-deficient mice: Biochemical basis for impaired vision. Biochemistry, 41(51), 15360-15368. https://doi.org/10.1021/bi0268551
Vogel, Silke ; Piantedosi, Roseann ; O'Byrne, Sheila M. ; Kako, Yuko ; Quadro, Loredana ; Gottesman, Max E. ; Goldberg, Ira J. ; Blaner, William S. / Retinol-binding protein-deficient mice : Biochemical basis for impaired vision. In: Biochemistry. 2002 ; Vol. 41, No. 51. pp. 15360-15368.
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Vogel, S, Piantedosi, R, O'Byrne, SM, Kako, Y, Quadro, L, Gottesman, ME, Goldberg, IJ & Blaner, WS 2002, 'Retinol-binding protein-deficient mice: Biochemical basis for impaired vision', Biochemistry, vol. 41, no. 51, pp. 15360-15368. https://doi.org/10.1021/bi0268551

Retinol-binding protein-deficient mice : Biochemical basis for impaired vision. / Vogel, Silke; Piantedosi, Roseann; O'Byrne, Sheila M.; Kako, Yuko; Quadro, Loredana; Gottesman, Max E.; Goldberg, Ira J.; Blaner, William S.

In: Biochemistry, Vol. 41, No. 51, 24.12.2002, p. 15360-15368.

Research output: Contribution to journalArticle

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T1 - Retinol-binding protein-deficient mice

T2 - Biochemical basis for impaired vision

AU - Vogel, Silke

AU - Piantedosi, Roseann

AU - O'Byrne, Sheila M.

AU - Kako, Yuko

AU - Quadro, Loredana

AU - Gottesman, Max E.

AU - Goldberg, Ira J.

AU - Blaner, William S.

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Vogel S, Piantedosi R, O'Byrne SM, Kako Y, Quadro L, Gottesman ME et al. Retinol-binding protein-deficient mice: Biochemical basis for impaired vision. Biochemistry. 2002 Dec 24;41(51):15360-15368. https://doi.org/10.1021/bi0268551