Reverse genetics and recombination in Φ8, a dsRNA bacteriophage

Shiroh Onodera, Yang Sun, Leonard Mindich

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Bacteriophage Φ8 has a genome of three dsRNA segments. It is able to acquire plasmid transcripts of cDNA copies of the genomic segments as replacements of its resident chromosomes. It is also able to effect recombination between the plasmid transcripts and the resident chromosomes. Depending upon the extent of sequence identity between the plasmid transcript and the resident chromosome, the recombination can be homologous or heterologous. Homologous recombination has not previously been reported for viruses with double-stranded RNA genomes.

Original languageEnglish (US)
Pages (from-to)113-118
Number of pages6
JournalVirology
Volume286
Issue number1
DOIs
StatePublished - Jul 20 2001

All Science Journal Classification (ASJC) codes

  • Virology

Keywords

  • Bacteriophage
  • Pseudomonas
  • Recombination
  • dsRNA

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