Rfc5, a small subunit of replication factor C complex, couples DNA replication and mitosis in budding yeast

Katsunori Sugimoto, Toshiyasu Shimomura, Keiji Hashimoto, Hiroyuki Araki, Akio Sugino, Kunihiro Matsumoto

Research output: Contribution to journalArticlepeer-review

81 Scopus citations

Abstract

The inhibition of DNA synthesis prevents mitotic entry through the action of the S phase checkpoint. In the yeast Saccharomyces cerevisiae, an essential protein kinase, Spk1/Mec2/Rad53/Sad1, controls the coupling of S phase to mitosis. In an attempt to identify genes that genetically interact with Spk1, we have isolated a temperature-sensitive mutation, rfc5-1, that can be suppressed by overexpression of SPK1. The RFC5 gene encodes a small subunit of replication factor C complex. At the restrictive temperature, rfc5-1 mutant cells entered mitosis with unevenly separated or fragmented chromosomes, resulting in loss of viability. Thus, the rfc5 mutation defective for DNA replication is also impaired in the S phase checkpoint. Overexpression of POL30, which encodes the proliferating cell nuclear antigen, suppressed the replication defect of the rfc5 mutant but not its checkpoint defect. Taken together, these results suggested that replication factor C has a direct role in sensing the state of DNA replication and transmitting the signal to the checkpoint machinery.

Original languageEnglish (US)
Pages (from-to)7048-7052
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number14
DOIs
StatePublished - Jul 9 1996

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • SPK1/MEC2/RAD53/SAD1
  • checkpoint control
  • proliferating cell nuclear antigen

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