Ribosome-controlled transcription termination is essential for the production of antibiotic microcin C

Inna Zukher, Maria Novikova, Anton Tikhonov, Mikhail V. Nesterchuk, Ilya A. Osterman, Marko Djordjevic, Petr V. Sergiev, Cynthia M. Sharma, Konstantin Severinov

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Microcin C (McC) is a peptide-nucleotide antibiotic produced by Escherichia coli cells harboring a plasmid-borne operon mccABCDE. The heptapeptide MccA is converted into McC by adenylation catalyzed by the MccB enzyme. Since MccA is a substrate for MccB, a mechanism that regulates the MccA/MccB ratio likely exists. Here, we show that transcription from a promoter located upstream of mccA directs the synthesis of two transcripts: a short highly abundant transcript containing the mccA ORF and a longer minor transcript containing mccA and downstream ORFs. The short transcript is generated when RNA polymerase terminates transcription at an intrinsic terminator located in the intergenic region between the mccA and mccB genes. The function of this terminator is strongly attenuated by upstream mcc sequences. Attenuation is relieved and transcription termination is induced when ribosome binds to the mccA ORF. Ribosome binding also makes the mccA RNA exceptionally stable. Together, these two effects-ribosomeinduced transcription termination and stabilization of themessage-account for very high abundance of the mccA transcript that is essential for McC production. The general scheme appears to be evolutionary conserved as ribosome-induced transcription termination also occurs in a homologous operon fromHelicobacter pylori.

Original languageEnglish (US)
Pages (from-to)11891-11902
Number of pages12
JournalNucleic acids research
Volume42
Issue number19
DOIs
StatePublished - Oct 29 2014

All Science Journal Classification (ASJC) codes

  • Genetics

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