Rif1 provides a new DNA-binding interface for the Bloom syndrome complex to maintain normal replication

Dongyi Xu, Parameswary Muniandy, Elisabetta Leo, Jinhu Yin, Saravanabhavan Thangavel, Xi Shen, Miki Ii, Keli Agama, Rong Guo, David Fox, Amom Ruhikanta Meetei, Lauren Wilson, Huy Nguyen, Nan Ping Weng, Steven J. Brill, Lei Li, Alessandro Vindigni, Yves Pommier, Michael Seidman, Weidong Wang

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

BLM, the helicase defective in Bloom syndrome, is part of a multiprotein complex that protects genome stability. Here, we show that Rif1 is a novel component of the BLM complex and works with BLM to promote recovery of stalled replication forks. First, Rif1 physically interacts with the BLM complex through a conserved C-terminal domain, and the stability of Rif1 depends on the presence of the BLM complex. Second, Rif1 and BLM are recruited with similar kinetics to stalled replication forks, and the Rif1 recruitment is delayed in BLM-deficient cells. Third, genetic analyses in vertebrate DT40 cells suggest that BLM and Rif1 work in a common pathway to resist replication stress and promote recovery of stalled forks. Importantly, vertebrate Rif1 contains a DNA-binding domain that resembles the Î ±CTD domain of bacterial RNA polymerase Î ±; and this domain preferentially binds fork and Holliday junction (HJ) DNA in vitro and is required for Rif1 to resist replication stress in vivo. Our data suggest that Rif1 provides a new DNA-binding interface for the BLM complex to restart stalled replication forks.

Original languageEnglish (US)
Pages (from-to)3140-3155
Number of pages16
JournalEMBO Journal
Volume29
Issue number18
DOIs
StatePublished - Sep 1 2010

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Keywords

  • BLM
  • Bloom syndrome
  • RMI
  • Rif1
  • replication

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  • Cite this

    Xu, D., Muniandy, P., Leo, E., Yin, J., Thangavel, S., Shen, X., Ii, M., Agama, K., Guo, R., Fox, D., Meetei, A. R., Wilson, L., Nguyen, H., Weng, N. P., Brill, S. J., Li, L., Vindigni, A., Pommier, Y., Seidman, M., & Wang, W. (2010). Rif1 provides a new DNA-binding interface for the Bloom syndrome complex to maintain normal replication. EMBO Journal, 29(18), 3140-3155. https://doi.org/10.1038/emboj.2010.186