@article{3afa5494a2a7448cb220be26a39cc590,
title = "RNA-Sequencing Analysis Reveals l -Theanine Regulating Transcriptional Rhythm Alteration in Vascular Smooth Muscle Cells Induced by Dexamethasone",
abstract = "l-Theanine, a unique amino acid in tea leaves, is known to have beneficial effects on stress relief, tumor suppression, and prevention of hypertension and cardiovascular diseases (CADs). The disruption of the circadian rhythm has been implied in the pathogenesis of CADs. However, it is unknown whether l-theanine has a modulatory effect on the vascular circadian rhythm. In this research, we have established a circadian gene expression model in rat vascular smooth muscle cells by dexamethasone induction. l-Theanine treatment enhanced the expression amplitude of clock genes, including Bmal1, Cry1, Rev-erbα, and Per2. Moreover, pairwise comparisons of the RNA-sequencing data showed that l-theanine is able to upregulate a ray of the rhythm genes and differentially expressed genes that are involved in vasoconstriction and actin cytoskeleton regulation pathways. Our data suggest that l-theanine changes the circadian gene rhythm involving in the process of vascular smooth muscle restructure.",
keywords = "RNA-seq, VSMCs, biological rhythms, circadian genes, l -theanine",
author = "Ruru Wang and Menchao Xiao and Yujing Zhang and Ho, {Chi Tang} and Xiaochun Wan and Daxiang Li and Zhongwen Xie",
note = "Funding Information: *Telephone: +86-551-65786153. E-mail: dxli@ahau.edu.cn. *Telephone: +86-551-65786153. E-mail: zhongwenxie@ahau. edu.cn. ORCID Zhongwen Xie: 0000-0002-3080-4881 Author Contributions Ruru Wang performed the experiments, collected data, and prepared the manuscript draft. Menchao Xiao and Yujing Zhang performed the experiments. Chi-Tang Ho and Xiaochun Wan were involved in directing the research goals and editing the manuscript. Daxiang Li and Zhongwen Xie were responsible for experimental design, analyzing data, and writing and editing the manuscript. Funding This research was funded by the National Sciences Foundation of China to Zhongwen Xie (Grant 31571207), a key grant from the Department of Sciences and Technology of Anhui Province to Zhongwen Xie (Grant 17030701017), a grant for Nutrition and Quality & Safety of Agricultural Products from the Anhui Province Talent Team Program to Zhongwen Xie, a grant for Anhui Featured Agricultural Development Project (Grant 2016-188), and a grant for supporting animal core facility in Anhui Agricultural University from the Department of Sciences and Technology of Anhui Province. Notes The authors declare no competing financial interest. Publisher Copyright: {\textcopyright} 2019 American Chemical Society.",
year = "2019",
month = may,
day = "15",
doi = "10.1021/acs.jafc.8b05057",
language = "English (US)",
volume = "67",
pages = "5413--5422",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "19",
}