@inbook{539b916198e04e87b9f55ecc934a1f84,
title = "RNA Sequencing Protocols for Short-Read Sequencing",
abstract = "RNA sequencing (RNA-seq) methodologies allow the discovery of novel variants and transcripts. These comprise three general steps: (1) capture of RNA species of interest, (2) conversion of RNA to complementary DNA (cDNA), and (3) modification of cDNA to fit the sequencing platform. Here we describe four different library preparation protocols for short-read sequencing: cDNA synthesis with poly(A) selection, library preparation with ribosomal depletion, and cDNA synthesis with SMART{\textregistered} (Switching Mechanism at 5′ end of RNA Template) technology for low and Pico inputs.",
keywords = "Library preparation, Poly(A) selection, RNA-seq, Ribosomal depletion, Short-read sequencing, cDNA",
author = "Laura Vasquez-Velez and Veera D{\textquoteright}Mello and Patricia Soteropoulos",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.",
year = "2025",
doi = "10.1007/978-1-0716-4192-7_8",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "125--158",
booktitle = "Methods in Molecular Biology",
}