Role of a novel coiled-coil domain-containing protein CCDC69 in regulating central spindle assembly

Debjani Pal, Di Wu, Akiko Haruta, Fumio Matsumura, Qize Wei

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


The formation of the central spindle (or the spindle midzone) is essential for cytokinesis in animal cells. In this study, we report that coiled-coil domain-containing protein 69 (CCDC69) is implicated in controlling the assembly of central spindles and the recruitment of midzone components. Exogenous expression of CCDC69 in HeLa cells interfered with microtubule polymerization and disrupted the formation of bipolar mitotic spindles. Endogenous CCDC69 proteins were localized to the central spindle during anaphase. RNA interference (RNAi)-mediated knockdown of CCDC69 led to the formation of aberrant central spindles and disrupted the localization of midzone components such as aurora B kinase, protein regulator of cytokinesis 1 (PRC1), MgcRacGAP/HsCYK-4, and polo-like kinase 1 (Plk1) at the central spindle. Aurora B kinase was found to bind to CCDC69 and this binding depended on the coiled-coil domains at the C-terminus of CCDC69. Further, disruption of aurora B function in HeLa cells by treatment with a small chemical inhibitor led to the mislocalization of CCDC69 at the central spindle. Our results indicate that CCDC69 acts as a scaffold to regulate the recruitment of midzone components and the assembly of central spindles.

Original languageEnglish (US)
Pages (from-to)4117-4129
Number of pages13
JournalCell Cycle
Issue number20
StatePublished - Oct 15 2010

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology


  • Aurora B
  • CCDC69
  • Central spindles
  • Cytokinesis
  • Midzone components
  • Plk1


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