Role of glutathione in macrophage control of mycobacteria

Vishwanath Venketaraman, Yaswant K. Dayaram, Amol G. Amin, Richard Ngo, Renee M. Green, Meliza T. Talaue, Jessica Mann, Nancy D. Connell

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Reactive oxygen and nitrogen intermediates are important antimicrobial defense mechanisms of macrophages and other phagocytic cells. While reactive nitrogen intermediates have been shown to play an important role in tuberculosis control in the murine system, their role in human disease is not clearly established. Glutathione, a tripeptide and antioxidant, is synthesized at high levels by cells during reactive oxygen intermediate and nitrogen intermediate production. Glutathione has been recently shown to play an important role in apoptosis and to regulate antigen-presenting-cell functions. Glutathione also serves as a carrier molecule for nitric oxide, in the form of S-nitrosoglutathione. Previous work from this laboratory has shown that glutathione and S-nitrosoglutathione are directly toxic to mycobacteria. A mutant strain of Mycobacterium bovis BCG, defective in the transport of small peptides such as glutathione, is resistant to the toxic effect of glutathione and S-nitrosoglutathione. Using the peptide transport mutant as a tool, we investigated the role of glutathione and S-nitrosoglutathione in animal and human macrophages in controlling intracellular mycobacterial growth.

Original languageEnglish (US)
Pages (from-to)1864-1871
Number of pages8
JournalInfection and immunity
Volume71
Issue number4
DOIs
StatePublished - Apr 1 2003

Fingerprint

Mycobacterium
Glutathione
S-Nitrosoglutathione
Macrophages
Nitrogen
Poisons
Mycobacterium bovis
Oxygen
Peptides
Antigen-Presenting Cells
Phagocytes
Nitric Oxide
Tuberculosis
Antioxidants
Apoptosis
Growth

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Venketaraman, V., Dayaram, Y. K., Amin, A. G., Ngo, R., Green, R. M., Talaue, M. T., ... Connell, N. D. (2003). Role of glutathione in macrophage control of mycobacteria. Infection and immunity, 71(4), 1864-1871. https://doi.org/10.1128/IAI.71.4.1864-1871.2003
Venketaraman, Vishwanath ; Dayaram, Yaswant K. ; Amin, Amol G. ; Ngo, Richard ; Green, Renee M. ; Talaue, Meliza T. ; Mann, Jessica ; Connell, Nancy D. / Role of glutathione in macrophage control of mycobacteria. In: Infection and immunity. 2003 ; Vol. 71, No. 4. pp. 1864-1871.
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Venketaraman, V, Dayaram, YK, Amin, AG, Ngo, R, Green, RM, Talaue, MT, Mann, J & Connell, ND 2003, 'Role of glutathione in macrophage control of mycobacteria', Infection and immunity, vol. 71, no. 4, pp. 1864-1871. https://doi.org/10.1128/IAI.71.4.1864-1871.2003

Role of glutathione in macrophage control of mycobacteria. / Venketaraman, Vishwanath; Dayaram, Yaswant K.; Amin, Amol G.; Ngo, Richard; Green, Renee M.; Talaue, Meliza T.; Mann, Jessica; Connell, Nancy D.

In: Infection and immunity, Vol. 71, No. 4, 01.04.2003, p. 1864-1871.

Research output: Contribution to journalArticle

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Venketaraman V, Dayaram YK, Amin AG, Ngo R, Green RM, Talaue MT et al. Role of glutathione in macrophage control of mycobacteria. Infection and immunity. 2003 Apr 1;71(4):1864-1871. https://doi.org/10.1128/IAI.71.4.1864-1871.2003