Role of host protein glutaredoxin 3 in the control of transcription during bacteriophage Φ2954 infection

Jian Qiao, Xueying Qiao, Yang Sun, Leonard Mindich

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8 Scopus citations

Abstract

Bacteriophage Φ2954 contains three dsRNA genomic segments, designated L, M, and S.The RNA is located inside a core particle composed of multiple copies of a major structural protein, an RNA-dependent RNA polymerase, a hexameric NTPase, and an auxiliary protein. The core particle is covered by a shell of protein P8, and this structure is enclosed within a lipid-containing membrane. We have found that normal infection of the host Pseudomonas syringae is dependent on the action of a host protein, glutaredoxin 3 (GrxC). GrxC removes the P8 shell from the infecting particle and binds to the inner core. Removal of P8 activates the transcription of segments S and M, whereas binding of GrxC to the core particle activates the transcription of segment L. The differences in transcription behavior are due to the preference of the polymerase for G as the first base of the transcript. Transcripts of segments S and M begin with GCAA, whereas those of segment L begin with ACAA. The binding of GrxC to the particle results in changes in polymerase activity. Mutations resulting in independence of GrxC are found in the gene for protein P1, the major structural protein of the inner core particle.

Original languageEnglish (US)
Pages (from-to)6000-6004
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number13
DOIs
StatePublished - Mar 30 2010

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All Science Journal Classification (ASJC) codes

  • General

Keywords

  • GFP fusion
  • Temporal control
  • dsRNA

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