Role of SIP30 in the development and maintenance of peripheral nerve injury-induced neuropathic pain

Yu Qiu Zhang, Ning Guo, Guangdun Peng, Mei Han, Jeremy Raincrow, Chi hua Chiu, Lique M. Coolen, Robert J. Wenthold, Zhi Qi Zhao, Naihe Jing, Lei Yu

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Using the chronic constriction injury (CCI) model of neuropathic pain, we profiled gene expression in the rat spinal cord, and identified SIP30 as a gene whose expression was elevated after CCI. SIP30 was previously shown to interact with SNAP25, but whose function was otherwise unknown. We now show that in the spinal cord, SIP30 was present in the dorsal horn laminae where the peripheral nociceptive inputs first synapse, co-localizing with nociception-related neuropeptides CGRP and substance P. With the onset of neuropathic pain after CCI surgery, SIP30 mRNA and protein levels increased in the ipsilateral side of the spinal cord, suggesting a potential association between SIP30 and neuropathic pain. When CCI-upregulated SIP30 was inhibited by intrathecal antisense oligonucleotide administration, neuropathic pain was attenuated. This neuropathic pain-reducing effect was observed both during neuropathic pain onset following CCI, and after neuropathic pain was fully established, implicating SIP30 involvement in the development and maintenance phases of neuropathic pain. Using a secretion assay in PC12 cells, anti-SIP30 siRNA decreased the total pool of synaptic vesicles available for exocytosis, pointing to a potential function for SIP30. These results suggest a role of SIP30 in the development and maintenance of peripheral nerve injury-induced neuropathic pain.

Original languageEnglish (US)
Pages (from-to)130-140
Number of pages11
JournalPain
Volume146
Issue number1-2
DOIs
StatePublished - Nov 2009

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Anesthesiology and Pain Medicine

Keywords

  • Chronic constriction injury
  • Dorsal horn
  • Intrathecal
  • Neuropathic pain
  • SIP30
  • Spinal cord

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  • Cite this

    Zhang, Y. Q., Guo, N., Peng, G., Han, M., Raincrow, J., Chiu, C. H., Coolen, L. M., Wenthold, R. J., Zhao, Z. Q., Jing, N., & Yu, L. (2009). Role of SIP30 in the development and maintenance of peripheral nerve injury-induced neuropathic pain. Pain, 146(1-2), 130-140. https://doi.org/10.1016/j.pain.2009.07.011