Role of voltage-sensitive calcium channels in mitogenic stimulation of neuroblasts

David W. Pincus, Emanuel DiCicco-Bloom, Ira B. Black

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The present study examines the role of Ca2+ in the regulation of sympathetic neuroblast mitosis. Employing a fully defined neuroblast culture system, we previously found that insulin growth factors (IGFs), depolarization and vasoactive intestinal peptide (VIP) regulated precursor mitosis. We now report that Ca2+ entry via voltage-sensitive channels was required for depolarization-stimulated mitogenesis. Ca2+ channel blockade with nitrendipine completely inhibited the increase in [3H]thymidine incorporation elicited by depolarizing stimuli including 30 mM KCl and the Na+ channel agonist veratridine. However, Ca2+ channel activity was not involved in the stimulation of DNA synthesis by IGFs or VIP. Thus, neuroblast mitosis may be regulated by multiple intracellular as well as extracellular signals.

Original languageEnglish (US)
Pages (from-to)211-214
Number of pages4
JournalBrain research
Volume553
Issue number2
DOIs
StatePublished - Jul 12 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Keywords

  • Insulin
  • Insulin-like growth factor-1
  • Neuroblast
  • Neurogenesis
  • Nitrendipine
  • Somatomedin C
  • Vasoactive intestinal peptide

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