RUNX2 is overexpressed in melanoma cells and mediates their migration and invasion

Rajeev K. Boregowda; Oyenike O. Olabisi; Walid Abushahba; Byeong Seon Jeong; Keneshia K. Haenssen; Wenjin Chen; Marina Chekmareva; Ahmed Lasfar; David J. Foran; James S. Goydos; Karine A. Cohen-Solal

doi:10.1016/j.canlet.2014.03.011

RUNX2 is overexpressed in melanoma cells and mediates their migration and invasion

Rajeev K. Boregowda, Oyenike O. Olabisi, Walid Abushahba, Byeong Seon Jeong, Keneshia K. Haenssen, Wenjin Chen, Marina Chekmareva, Ahmed Lasfar, David J. Foran, James S. Goydos, Karine A. Cohen-Solal

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

In the present study, we investigated the role of the transcription factor RUNX2 in melanomagenesis. We demonstrated that the expression of transcriptionally active RUNX2 was increased in melanoma cell lines as compared with human melanocytes. Using a melanoma tissue microarray, we showed that RUNX2 levels were higher in melanoma cells as compared with nevic melanocytes. RUNX2 knockdown in melanoma cell lines significantly decreased Focal Adhesion Kinase expression, and inhibited their cell growth, migration and invasion ability. Finally, the pro-hormone cholecalciferol reduced RUNX2 transcriptional activity and decreased migration of melanoma cells, further suggesting a role of RUNX2 in melanoma cell migration.

Original language	English (US)
Pages (from-to)	61-70
Number of pages	10
Journal	Cancer Letters
Volume	348
Issue number	1-2
DOIs	https://doi.org/10.1016/j.canlet.2014.03.011
State	Published - 2014

All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

Keywords

Cholecalciferol (Vitamin D3)
FAK
Melanoma
Migration
RUNX2
Transcription factor

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10.1016/j.canlet.2014.03.011

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@article{489003e392d74ffa81e4743ec5a0477f,

title = "RUNX2 is overexpressed in melanoma cells and mediates their migration and invasion",

abstract = "In the present study, we investigated the role of the transcription factor RUNX2 in melanomagenesis. We demonstrated that the expression of transcriptionally active RUNX2 was increased in melanoma cell lines as compared with human melanocytes. Using a melanoma tissue microarray, we showed that RUNX2 levels were higher in melanoma cells as compared with nevic melanocytes. RUNX2 knockdown in melanoma cell lines significantly decreased Focal Adhesion Kinase expression, and inhibited their cell growth, migration and invasion ability. Finally, the pro-hormone cholecalciferol reduced RUNX2 transcriptional activity and decreased migration of melanoma cells, further suggesting a role of RUNX2 in melanoma cell migration.",

keywords = "Cholecalciferol (Vitamin D3), FAK, Melanoma, Migration, RUNX2, Transcription factor",

author = "Boregowda, {Rajeev K.} and Olabisi, {Oyenike O.} and Walid Abushahba and Jeong, {Byeong Seon} and Haenssen, {Keneshia K.} and Wenjin Chen and Marina Chekmareva and Ahmed Lasfar and Foran, {David J.} and Goydos, {James S.} and Cohen-Solal, {Karine A.}",

year = "2014",

doi = "10.1016/j.canlet.2014.03.011",

language = "English (US)",

volume = "348",

pages = "61--70",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "1-2",

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TY - JOUR

T1 - RUNX2 is overexpressed in melanoma cells and mediates their migration and invasion

AU - Boregowda, Rajeev K.

AU - Olabisi, Oyenike O.

AU - Abushahba, Walid

AU - Jeong, Byeong Seon

AU - Haenssen, Keneshia K.

AU - Chen, Wenjin

AU - Chekmareva, Marina

AU - Lasfar, Ahmed

AU - Foran, David J.

AU - Goydos, James S.

AU - Cohen-Solal, Karine A.

PY - 2014

Y1 - 2014

N2 - In the present study, we investigated the role of the transcription factor RUNX2 in melanomagenesis. We demonstrated that the expression of transcriptionally active RUNX2 was increased in melanoma cell lines as compared with human melanocytes. Using a melanoma tissue microarray, we showed that RUNX2 levels were higher in melanoma cells as compared with nevic melanocytes. RUNX2 knockdown in melanoma cell lines significantly decreased Focal Adhesion Kinase expression, and inhibited their cell growth, migration and invasion ability. Finally, the pro-hormone cholecalciferol reduced RUNX2 transcriptional activity and decreased migration of melanoma cells, further suggesting a role of RUNX2 in melanoma cell migration.

AB - In the present study, we investigated the role of the transcription factor RUNX2 in melanomagenesis. We demonstrated that the expression of transcriptionally active RUNX2 was increased in melanoma cell lines as compared with human melanocytes. Using a melanoma tissue microarray, we showed that RUNX2 levels were higher in melanoma cells as compared with nevic melanocytes. RUNX2 knockdown in melanoma cell lines significantly decreased Focal Adhesion Kinase expression, and inhibited their cell growth, migration and invasion ability. Finally, the pro-hormone cholecalciferol reduced RUNX2 transcriptional activity and decreased migration of melanoma cells, further suggesting a role of RUNX2 in melanoma cell migration.

KW - Cholecalciferol (Vitamin D3)

KW - FAK

KW - Melanoma

KW - Migration

KW - RUNX2

KW - Transcription factor

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U2 - 10.1016/j.canlet.2014.03.011

DO - 10.1016/j.canlet.2014.03.011

M3 - Article

C2 - 24657655

AN - SCOPUS:84900470421

SN - 0304-3835

VL - 348

SP - 61

EP - 70

JO - Cancer Letters

JF - Cancer Letters

IS - 1-2

ER -

RUNX2 is overexpressed in melanoma cells and mediates their migration and invasion

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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