Sarco(endo)plasmic reticulum Ca2+ ATPase isoforms and their role in muscle physiology and pathology

Evgeny Loukianov, J. I. Yong, Debra L. Baker, Thomas Reed, Jegadeesh Babu, Tanya Loukianova, Adam Greene, Gary Shull, Muthu Periasamy

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Recent studies suggest that SR Ca2+ transport function is altered in hypertrophied and failing myocardium. To understand whether alterations in SR Ca2+ ATPase levels affect myocardial contractility, we generated transgenic mice that specifically overexpress SERCA2a or SERCA1 pump in the mouse heart, using the cardiac α-MHC promoter. Analysis of SERCA2a transgenic mice show both an increase in mRNA and protein levels (120-150% of the wild type). Isolated work performing heart preparations revealed that SERCA2a mice have improved myocardial performance. On the other hand, SERCA1 overexpression in the heart resulted in isoform replacement without any change in total SERCA protein. Interestingly, SERCA1 transgenic hearts exhibited super contractility with a significant increase in rates of muscle contraction (+dp/dt) and relaxation (-dp/dT). The time to peak pressure and half-time to relaxation were significantly shorter.

Original languageEnglish (US)
Pages (from-to)251-259
Number of pages9
JournalAnnals of the New York Academy of Sciences
Volume853
DOIs
StatePublished - 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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