Sarco(endo)plasmic reticulum Ca2+ ATPase isoforms and their role in muscle physiology and pathology

Evgeny Loukianov; J. I. Yong; Debra L. Baker; Thomas Reed; Jegadeesh Babu; Tanya Loukianova; Adam Greene; Gary Shull; Muthu Periasamy

doi:10.1111/j.1749-6632.1998.tb08273.x

Sarco(endo)plasmic reticulum Ca²⁺ ATPase isoforms and their role in muscle physiology and pathology

Evgeny Loukianov, J. I. Yong, Debra L. Baker, Thomas Reed, Jegadeesh Babu, Tanya Loukianova, Adam Greene, Gary Shull, Muthu Periasamy

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Recent studies suggest that SR Ca²⁺ transport function is altered in hypertrophied and failing myocardium. To understand whether alterations in SR Ca²⁺ ATPase levels affect myocardial contractility, we generated transgenic mice that specifically overexpress SERCA2a or SERCA1 pump in the mouse heart, using the cardiac α-MHC promoter. Analysis of SERCA2a transgenic mice show both an increase in mRNA and protein levels (120-150% of the wild type). Isolated work performing heart preparations revealed that SERCA2a mice have improved myocardial performance. On the other hand, SERCA1 overexpression in the heart resulted in isoform replacement without any change in total SERCA protein. Interestingly, SERCA1 transgenic hearts exhibited super contractility with a significant increase in rates of muscle contraction (+dp/dt) and relaxation (-dp/dT). The time to peak pressure and half-time to relaxation were significantly shorter.

Original language	English (US)
Pages (from-to)	251-259
Number of pages	9
Journal	Annals of the New York Academy of Sciences
Volume	853
DOIs	https://doi.org/10.1111/j.1749-6632.1998.tb08273.x
State	Published - 1998
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
History and Philosophy of Science

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10.1111/j.1749-6632.1998.tb08273.x

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title = "Sarco(endo)plasmic reticulum Ca2+ ATPase isoforms and their role in muscle physiology and pathology",

abstract = "Recent studies suggest that SR Ca2+ transport function is altered in hypertrophied and failing myocardium. To understand whether alterations in SR Ca2+ ATPase levels affect myocardial contractility, we generated transgenic mice that specifically overexpress SERCA2a or SERCA1 pump in the mouse heart, using the cardiac α-MHC promoter. Analysis of SERCA2a transgenic mice show both an increase in mRNA and protein levels (120-150% of the wild type). Isolated work performing heart preparations revealed that SERCA2a mice have improved myocardial performance. On the other hand, SERCA1 overexpression in the heart resulted in isoform replacement without any change in total SERCA protein. Interestingly, SERCA1 transgenic hearts exhibited super contractility with a significant increase in rates of muscle contraction (+dp/dt) and relaxation (-dp/dT). The time to peak pressure and half-time to relaxation were significantly shorter.",

author = "Evgeny Loukianov and Yong, {J. I.} and Baker, {Debra L.} and Thomas Reed and Jegadeesh Babu and Tanya Loukianova and Adam Greene and Gary Shull and Muthu Periasamy",

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doi = "10.1111/j.1749-6632.1998.tb08273.x",

language = "English (US)",

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T1 - Sarco(endo)plasmic reticulum Ca2+ ATPase isoforms and their role in muscle physiology and pathology

AU - Loukianov, Evgeny

AU - Yong, J. I.

AU - Baker, Debra L.

AU - Reed, Thomas

AU - Babu, Jegadeesh

AU - Loukianova, Tanya

AU - Greene, Adam

AU - Shull, Gary

AU - Periasamy, Muthu

PY - 1998

Y1 - 1998

N2 - Recent studies suggest that SR Ca2+ transport function is altered in hypertrophied and failing myocardium. To understand whether alterations in SR Ca2+ ATPase levels affect myocardial contractility, we generated transgenic mice that specifically overexpress SERCA2a or SERCA1 pump in the mouse heart, using the cardiac α-MHC promoter. Analysis of SERCA2a transgenic mice show both an increase in mRNA and protein levels (120-150% of the wild type). Isolated work performing heart preparations revealed that SERCA2a mice have improved myocardial performance. On the other hand, SERCA1 overexpression in the heart resulted in isoform replacement without any change in total SERCA protein. Interestingly, SERCA1 transgenic hearts exhibited super contractility with a significant increase in rates of muscle contraction (+dp/dt) and relaxation (-dp/dT). The time to peak pressure and half-time to relaxation were significantly shorter.

AB - Recent studies suggest that SR Ca2+ transport function is altered in hypertrophied and failing myocardium. To understand whether alterations in SR Ca2+ ATPase levels affect myocardial contractility, we generated transgenic mice that specifically overexpress SERCA2a or SERCA1 pump in the mouse heart, using the cardiac α-MHC promoter. Analysis of SERCA2a transgenic mice show both an increase in mRNA and protein levels (120-150% of the wild type). Isolated work performing heart preparations revealed that SERCA2a mice have improved myocardial performance. On the other hand, SERCA1 overexpression in the heart resulted in isoform replacement without any change in total SERCA protein. Interestingly, SERCA1 transgenic hearts exhibited super contractility with a significant increase in rates of muscle contraction (+dp/dt) and relaxation (-dp/dT). The time to peak pressure and half-time to relaxation were significantly shorter.

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ER -

Sarco(endo)plasmic reticulum Ca²⁺ ATPase isoforms and their role in muscle physiology and pathology

Abstract

All Science Journal Classification (ASJC) codes

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