The reaction between aromatic amines and aromatic aldehydes was investigated as the basis of a spectrophotometric assay for sulfonamides. Those properties of the equilibrium reaction which affect the absorptimetric properties of the system were studied to evaluate the method. The effects of pH, ionic strength, concentration of aldehyde, solvent system, and temperature were determined. The method was applied to the determination of sulfanilamide, sulfadimethoxine, and succinylsulfathiazole, and comparative analyses were performed with the method of Bratton and Marshall. The low absorbance method of precision spectrophotometry was employed in the determination of microgram quantities of sulfanilamide.
All Science Journal Classification (ASJC) codes
- Pharmaceutical Science