SECONDARY METABOLITES FROM ORGANIZED ATROPA BELLADONNA CULTURES.

Robert M. Hamilton, Jeffrey Lang, Henrik Pedersen, Chee Kok Chin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

Regenerated root cultures of Atropa belladonna (deadly nightshade) are used in an efficient format for the production of tropane alkaloids. Shake flasks were employed to convert selected, embryogenic callus lines to embryos that in turn form roots. Tropane alkaloids were found only in association with the roots, reaching about 0. 2% of the dry weight. A novel membrane-based culture system in the form of small 'boats' enables continuous callus cultivation for semicontinuous operation.

Original languageEnglish (US)
Title of host publicationBiotechnology and Bioengineering Symposium
EditorsCharles D. Scott
PublisherJohn Wiley & Sons
Pages685-698
Number of pages14
Edition17
ISBN (Print)0471627623
StatePublished - Dec 1 1986

Publication series

NameBiotechnology and Bioengineering Symposium
Number17
ISSN (Print)0572-6565

Fingerprint

Atropa belladonna
Tropanes
Bony Callus
Metabolites
Alkaloids
Solanum
Ships
Boats
Embryonic Structures
Membranes
Weights and Measures

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Hamilton, R. M., Lang, J., Pedersen, H., & Chin, C. K. (1986). SECONDARY METABOLITES FROM ORGANIZED ATROPA BELLADONNA CULTURES. In C. D. Scott (Ed.), Biotechnology and Bioengineering Symposium (17 ed., pp. 685-698). (Biotechnology and Bioengineering Symposium; No. 17). John Wiley & Sons.
Hamilton, Robert M. ; Lang, Jeffrey ; Pedersen, Henrik ; Chin, Chee Kok. / SECONDARY METABOLITES FROM ORGANIZED ATROPA BELLADONNA CULTURES. Biotechnology and Bioengineering Symposium. editor / Charles D. Scott. 17. ed. John Wiley & Sons, 1986. pp. 685-698 (Biotechnology and Bioengineering Symposium; 17).
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Hamilton, RM, Lang, J, Pedersen, H & Chin, CK 1986, SECONDARY METABOLITES FROM ORGANIZED ATROPA BELLADONNA CULTURES. in CD Scott (ed.), Biotechnology and Bioengineering Symposium. 17 edn, Biotechnology and Bioengineering Symposium, no. 17, John Wiley & Sons, pp. 685-698.

SECONDARY METABOLITES FROM ORGANIZED ATROPA BELLADONNA CULTURES. / Hamilton, Robert M.; Lang, Jeffrey; Pedersen, Henrik; Chin, Chee Kok.

Biotechnology and Bioengineering Symposium. ed. / Charles D. Scott. 17. ed. John Wiley & Sons, 1986. p. 685-698 (Biotechnology and Bioengineering Symposium; No. 17).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Hamilton, Robert M.

AU - Lang, Jeffrey

AU - Pedersen, Henrik

AU - Chin, Chee Kok

PY - 1986/12/1

Y1 - 1986/12/1

N2 - Regenerated root cultures of Atropa belladonna (deadly nightshade) are used in an efficient format for the production of tropane alkaloids. Shake flasks were employed to convert selected, embryogenic callus lines to embryos that in turn form roots. Tropane alkaloids were found only in association with the roots, reaching about 0. 2% of the dry weight. A novel membrane-based culture system in the form of small 'boats' enables continuous callus cultivation for semicontinuous operation.

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Hamilton RM, Lang J, Pedersen H, Chin CK. SECONDARY METABOLITES FROM ORGANIZED ATROPA BELLADONNA CULTURES. In Scott CD, editor, Biotechnology and Bioengineering Symposium. 17 ed. John Wiley & Sons. 1986. p. 685-698. (Biotechnology and Bioengineering Symposium; 17).