Selective activation of the "b" splice variant of phospholipase Cβ1 in chronically dilated human and mouse atria

Elizabeth A. Woodcock, David R. Grubb, Theresa M. Filtz, Silvana Marasco, Jieting Luo, Tiffany J. McLeod-Dryden, David M. Kaye, Junichi Sadoshima, Xiao Jun Du, Chiew Wong, Julie R. McMullen, Anthony M. Dart

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Atrial fibrillation (AF) is commonly associated with chronic dilatation of the left atrium, both in human disease and animal models. The immediate signaling enzyme phospholipase C (PLC) is activated by mechanical stretch to generate the Ca2+-releasing messenger inositol(1,4,5)trisphosphate (Ins(1,4,5)P3) and sn-1,2-diacylglycerol (DAG), an activator of protein kinase C subtypes. There is also evidence that heightened activity of PLC, caused by the receptor coupling protein Gq, can contribute to atrial remodelling. We examined PLC activation in right and left atrial appendage from patients with mitral valve disease (VHD) and in a mouse model of dilated cardiomyopathy caused by transgenic overexpression of the stress-activated protein kinase, mammalian sterile 20 like kinase 1 (Mst1) (Mst1-TG). PLC activation was heightened 6- to 10-fold in atria from VHD patients compared with right atrial tissue from patients undergoing coronary artery bypass surgery (CABG) and was also heightened in the dilated atria from Mst1-TG. PLC activation in human left atrial appendage and in mouse left atria correlated with left atrial size, implying a relationship between PLC activation and chronic dilatation. Dilated atria from human and mouse showed heightened expression of PLCβ1b, but not of other PLC subtypes. PLCβ1b, but not PLCβ1a, caused apoptosis when overexpressed in neonatal rat cardiomyocytes, suggesting that PLCβ1b may contribute to chamber dilatation. The activation of PLCβ1b is a possible therapeutic target to limit atrial remodelling in VHD patients.

Original languageEnglish (US)
Pages (from-to)676-683
Number of pages8
JournalJournal of Molecular and Cellular Cardiology
Volume47
Issue number5
DOIs
StatePublished - Nov 2009

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Keywords

  • Atrial fibrillation
  • Atrial myocyte
  • G proteins
  • Norepinephrine
  • Signaling pathway
  • Stretch

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