Abstract
For decades, it has been thought that glutamate and GABA are released by distinct neurons. However, some mouse neurons innervating the lateral habenula (LHb) co-release glutamate and GABA. Here, we mapped the distribution of neurons throughout the rat brain that co-express vesicular transporters for the accumulation of glutamate (VGluT2) or GABA (VGaT) and for GABA synthesis (GAD). We found concentrated groups of neurons that co-express VGluT2, VGaT, and GAD mRNAs within subdivisions of the ventral tegmental area (VTA), entopeduncular (EPN), and supramammillary (SUM) nuclei. Single axon terminals established by VTA, EPN, or SUM neurons form a common synaptic architecture involving asymmetric (putative excitatory) and symmetric (putative inhibitory) synapses. Within the LHb, which receives co-transmitted glutamate and GABA from VTA and EPN, VGluT2 and VGaT are distributed on separate synaptic vesicles. We conclude that single axon terminals from VGluT2 and VGaT co-expressing neurons co-transmit glutamate and GABA from distinct synaptic vesicles at independent synapses. Root et al. identify concentrated populations of glutamate and GABA co-transmitting neurons in VTA, SUM, and EPN. Single axon terminals from these neurons form a common synaptic architecture that co-transmits glutamate and GABA from distinct synaptic vesicles at independent asymmetric or symmetric synapses.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3465-3479 |
| Number of pages | 15 |
| Journal | Cell Reports |
| Volume | 23 |
| Issue number | 12 |
| DOIs | |
| State | Published - Jun 19 2018 |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
Keywords
- GABA
- GABA-glutamate co-release
- co-transmission
- entopeduncular nucleus
- glutamate
- hippocampus
- lateral habenula
- supramammillary nucleus
- ventral tegmental area
Cite this
}
Selective Brain Distribution and Distinctive Synaptic Architecture of Dual Glutamatergic-GABAergic Neurons. / Root, David H.; Zhang, Shiliang; Barker, David; Miranda-Barrientos, Jorge; Liu, Bing; Wang, Hui Ling; Morales, Marisela.
In: Cell Reports, Vol. 23, No. 12, 19.06.2018, p. 3465-3479.Research output: Contribution to journal › Article
TY - JOUR
T1 - Selective Brain Distribution and Distinctive Synaptic Architecture of Dual Glutamatergic-GABAergic Neurons
AU - Root, David H.
AU - Zhang, Shiliang
AU - Barker, David
AU - Miranda-Barrientos, Jorge
AU - Liu, Bing
AU - Wang, Hui Ling
AU - Morales, Marisela
PY - 2018/6/19
Y1 - 2018/6/19
N2 - For decades, it has been thought that glutamate and GABA are released by distinct neurons. However, some mouse neurons innervating the lateral habenula (LHb) co-release glutamate and GABA. Here, we mapped the distribution of neurons throughout the rat brain that co-express vesicular transporters for the accumulation of glutamate (VGluT2) or GABA (VGaT) and for GABA synthesis (GAD). We found concentrated groups of neurons that co-express VGluT2, VGaT, and GAD mRNAs within subdivisions of the ventral tegmental area (VTA), entopeduncular (EPN), and supramammillary (SUM) nuclei. Single axon terminals established by VTA, EPN, or SUM neurons form a common synaptic architecture involving asymmetric (putative excitatory) and symmetric (putative inhibitory) synapses. Within the LHb, which receives co-transmitted glutamate and GABA from VTA and EPN, VGluT2 and VGaT are distributed on separate synaptic vesicles. We conclude that single axon terminals from VGluT2 and VGaT co-expressing neurons co-transmit glutamate and GABA from distinct synaptic vesicles at independent synapses. Root et al. identify concentrated populations of glutamate and GABA co-transmitting neurons in VTA, SUM, and EPN. Single axon terminals from these neurons form a common synaptic architecture that co-transmits glutamate and GABA from distinct synaptic vesicles at independent asymmetric or symmetric synapses.
AB - For decades, it has been thought that glutamate and GABA are released by distinct neurons. However, some mouse neurons innervating the lateral habenula (LHb) co-release glutamate and GABA. Here, we mapped the distribution of neurons throughout the rat brain that co-express vesicular transporters for the accumulation of glutamate (VGluT2) or GABA (VGaT) and for GABA synthesis (GAD). We found concentrated groups of neurons that co-express VGluT2, VGaT, and GAD mRNAs within subdivisions of the ventral tegmental area (VTA), entopeduncular (EPN), and supramammillary (SUM) nuclei. Single axon terminals established by VTA, EPN, or SUM neurons form a common synaptic architecture involving asymmetric (putative excitatory) and symmetric (putative inhibitory) synapses. Within the LHb, which receives co-transmitted glutamate and GABA from VTA and EPN, VGluT2 and VGaT are distributed on separate synaptic vesicles. We conclude that single axon terminals from VGluT2 and VGaT co-expressing neurons co-transmit glutamate and GABA from distinct synaptic vesicles at independent synapses. Root et al. identify concentrated populations of glutamate and GABA co-transmitting neurons in VTA, SUM, and EPN. Single axon terminals from these neurons form a common synaptic architecture that co-transmits glutamate and GABA from distinct synaptic vesicles at independent asymmetric or symmetric synapses.
KW - GABA
KW - GABA-glutamate co-release
KW - co-transmission
KW - entopeduncular nucleus
KW - glutamate
KW - hippocampus
KW - lateral habenula
KW - supramammillary nucleus
KW - ventral tegmental area
UR - http://www.scopus.com/inward/record.url?scp=85048335213&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85048335213&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2018.05.063
DO - 10.1016/j.celrep.2018.05.063
M3 - Article
AN - SCOPUS:85048335213
VL - 23
SP - 3465
EP - 3479
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 12
ER -