Selenite prevents the development of drug resistance in tumor cells in vitro

Gerald Frenkel, Paula B. Caffrey, Ming Zhu

Research output: Contribution to journalArticle

Abstract

The development of drug resistance is the primary reason for the failure of chemotherapy as a treatment for human cancer. We have been investigating the potential applications of selenium compounds in the solution of this problem. In order to examine whether selenite can interfere with the development of drug resistance, we have designed a model system in which exposure of drug-sensitive A2780 human ovarian tumor cells to low concentrations of melphalan for 7 days results in a population of drug-resistant cells. These cells have higher levels of glutathione-S-transferase (GST) activity than the drug-sensitive parent cells; exposure of the cells to ethacrynic arid decreased their level of GST activity and also eliminated their resistance to melphalan. The inclusion of selenite during the melphalan exposure (at concentrations as low as 200 nM) effectively prevented the development of drug resistance, probably by preventing the melphalan-induced increase in GST activity. These results provide the first indication that selenite may be a useful agent for preventing the development of drug resistance during cancer chemotherapy.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - Dec 1 1997

Fingerprint

Selenious Acid
selenites
drug resistance
Drug Resistance
Melphalan
Tumors
glutathione transferase
Cells
Glutathione Transferase
drugs
Pharmaceutical Preparations
drug therapy
Neoplasms
cells
Chemotherapy
neoplasms
ovarian neoplasms
Selenium Compounds
Drug Therapy
selenium

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

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Selenite prevents the development of drug resistance in tumor cells in vitro. / Frenkel, Gerald; Caffrey, Paula B.; Zhu, Ming.

In: FASEB Journal, Vol. 11, No. 3, 01.12.1997.

Research output: Contribution to journalArticle

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