Selenotrisulfide inhibits initiation by RNA polymerase II but not elongation

Gerald D. Frenkel, Darlene Falvey

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6 Scopus citations

Abstract

We previously reported that RNA polymerase II (purified from wheat germ) is inhibited by selenotrisulfides, the products of the reaction of selenite with sulfhydryl compounds [Frenkel, Walcott, and Middleton, Molecular Pharmacology 31, 112 (1987)]. We have now found that the initiation stage of the reaction is inhibited by selenotrisulfide but the elongation stage of the reaction is not. The actual start of the RNA chain is not inhibited by the selenotrisulfide, but rather the formation of the enzyme- DNA binary complex. Selenotrisulfide has a similar differential effect on initiation and elongation by RNA polymerase II from HeLa cells; in contrast, with E. coli RNA polymerase, it inhibits elongation as well.

Original languageEnglish (US)
Pages (from-to)179-182
Number of pages4
JournalJournal of Inorganic Biochemistry
Volume35
Issue number3
DOIs
StatePublished - Mar 1989

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Inorganic Chemistry

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