TY - JOUR
T1 - Sensitivity of human colon tumor metastases to anticancer drugs in athymic (nude) mice
AU - Zirvi, Karimullah A.
AU - Najjar, Talib A.
AU - Slomiany, Bronislaw L.
N1 - Funding Information:
This work was supportedb y NIH grants no. DK21684-15a nd no. AA05858-10a nd a BRSG grant.
PY - 1993/8/16
Y1 - 1993/8/16
N2 - We have studied the metastatic behavior of five human colon tumor cell lines (LS174T, WiDr, Caco-2, SW 620 and SW 480) using intrasplenicnude mouse (ISMS) and intravenous-nude mouse (IVMS) model systems. LS174T was highly metastatic in both systems. In the IVMS system, LS174T cells produced lung metastasis and also grew in the skin as if the cells had been injected subcutaneously. We have also studied the antimetastatic activity of three anticancer drugs (5-fluorouracil (5-FU), doxorubicin (DX) and 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) using LS174T cells and both ISMS and IVMS systems. The drugs were given intravenously on day 19 and 26 of tumor cell injection. Mice were sacrificed and organs observed for metastatic growth 4-6 weeks after cell injection. The results show that in the ISMS system, BCNU and 5-FU are inactive against both the liver metastasis and primary growth in the spleen. DX inhibits metastatic growth but not the primary growth. In the IVMS system, BCNU is inactive, whereas 5-FU and DX are active against the metastatic growth. Thus, DX may have activity against blood-borne human colon tumor metastasis.
AB - We have studied the metastatic behavior of five human colon tumor cell lines (LS174T, WiDr, Caco-2, SW 620 and SW 480) using intrasplenicnude mouse (ISMS) and intravenous-nude mouse (IVMS) model systems. LS174T was highly metastatic in both systems. In the IVMS system, LS174T cells produced lung metastasis and also grew in the skin as if the cells had been injected subcutaneously. We have also studied the antimetastatic activity of three anticancer drugs (5-fluorouracil (5-FU), doxorubicin (DX) and 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) using LS174T cells and both ISMS and IVMS systems. The drugs were given intravenously on day 19 and 26 of tumor cell injection. Mice were sacrificed and organs observed for metastatic growth 4-6 weeks after cell injection. The results show that in the ISMS system, BCNU and 5-FU are inactive against both the liver metastasis and primary growth in the spleen. DX inhibits metastatic growth but not the primary growth. In the IVMS system, BCNU is inactive, whereas 5-FU and DX are active against the metastatic growth. Thus, DX may have activity against blood-borne human colon tumor metastasis.
KW - antimetastic drugs
KW - human colon tumor
KW - intrasplenic-nude mouse model system
KW - metastasis
UR - http://www.scopus.com/inward/record.url?scp=0027216397&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027216397&partnerID=8YFLogxK
U2 - 10.1016/0304-3835(93)90008-W
DO - 10.1016/0304-3835(93)90008-W
M3 - Article
C2 - 8402572
AN - SCOPUS:0027216397
VL - 72
SP - 39
EP - 44
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 1-2
ER -