Separation of substituted pteroyl monoglutamates and pteroyl oligo-γ-l-glutamates by high pressure liquid chromatography

R. W. Stout; A. R. Cashmore; J. K. Coward; C. G. Horvath; J. R. Bertino

doi:10.1016/0003-2697(76)90017-8

Separation of substituted pteroyl monoglutamates and pteroyl oligo-γ-l-glutamates by high pressure liquid chromatography

R. W. Stout, A. R. Cashmore, J. K. Coward, C. G. Horvath, J. R. Bertino

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Rapid analysis of substituted and unsubstituted pteroyl-oligo-γ-l-glutamates at the nanomole level is carried out by high performance liquid chromatography. The use of a siliceous microparticulate anion-exchanger column and gradient elution at pH 6.5 with increasing salt concentration facilitates the separation of the species containing up to seven glutamyl residues without decomposition in 30 min. The column effluent is monitored with a uv detector at 254 nm, and the peaks are conveniently identified by their retention.

Original language	English (US)
Pages (from-to)	119-124
Number of pages	6
Journal	Analytical Biochemistry
Volume	71
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(76)90017-8
State	Published - Mar 1976
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(76)90017-8

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title = "Separation of substituted pteroyl monoglutamates and pteroyl oligo-γ-l-glutamates by high pressure liquid chromatography",

abstract = "Rapid analysis of substituted and unsubstituted pteroyl-oligo-γ-l-glutamates at the nanomole level is carried out by high performance liquid chromatography. The use of a siliceous microparticulate anion-exchanger column and gradient elution at pH 6.5 with increasing salt concentration facilitates the separation of the species containing up to seven glutamyl residues without decomposition in 30 min. The column effluent is monitored with a uv detector at 254 nm, and the peaks are conveniently identified by their retention.",

author = "Stout, {R. W.} and Cashmore, {A. R.} and Coward, {J. K.} and Horvath, {C. G.} and Bertino, {J. R.}",

note = "Funding Information: 1 Supported by Grant #CA-10748, USPHS. Grant #CA-08010, USPHS, Grant #MH- 18038, USPHS, and Grant #9M20993. NIH, USPHS. 5 Author to whom requests for reprints should be addressed.",

year = "1976",

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doi = "10.1016/0003-2697(76)90017-8",

language = "English (US)",

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pages = "119--124",

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T1 - Separation of substituted pteroyl monoglutamates and pteroyl oligo-γ-l-glutamates by high pressure liquid chromatography

AU - Stout, R. W.

AU - Cashmore, A. R.

AU - Coward, J. K.

AU - Horvath, C. G.

AU - Bertino, J. R.

N1 - Funding Information: 1 Supported by Grant #CA-10748, USPHS. Grant #CA-08010, USPHS, Grant #MH- 18038, USPHS, and Grant #9M20993. NIH, USPHS. 5 Author to whom requests for reprints should be addressed.

PY - 1976/3

Y1 - 1976/3

N2 - Rapid analysis of substituted and unsubstituted pteroyl-oligo-γ-l-glutamates at the nanomole level is carried out by high performance liquid chromatography. The use of a siliceous microparticulate anion-exchanger column and gradient elution at pH 6.5 with increasing salt concentration facilitates the separation of the species containing up to seven glutamyl residues without decomposition in 30 min. The column effluent is monitored with a uv detector at 254 nm, and the peaks are conveniently identified by their retention.

AB - Rapid analysis of substituted and unsubstituted pteroyl-oligo-γ-l-glutamates at the nanomole level is carried out by high performance liquid chromatography. The use of a siliceous microparticulate anion-exchanger column and gradient elution at pH 6.5 with increasing salt concentration facilitates the separation of the species containing up to seven glutamyl residues without decomposition in 30 min. The column effluent is monitored with a uv detector at 254 nm, and the peaks are conveniently identified by their retention.

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Separation of substituted pteroyl monoglutamates and pteroyl oligo-γ-l-glutamates by high pressure liquid chromatography

Abstract

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