Sequence analysis of the variable number tandem repeat in Staphylococcus aureus protein a gene: Spa Typing

Barun Mathema, Jose Mediavilla, Barry N. Kreiswirth

Research output: Chapter in Book/Report/Conference proceedingChapter

44 Scopus citations

Abstract

The analyses of numerous prokaryotic and eukaryotic genomes have revealed the presence of variable number tandem repeats (VNTRs). VNTR analysis is currently widely used to sub-speciate many bacterial, fungal, and viral pathogens and has facilitated a number of molecular epidemiology studies. In this chapter, we focus on spa typing which is based on sequence analysis of VNTRs in the polymorphic X region of the Staphylococcus aureus protein A gene Staphylococcus aureus. As the specific methods for spa typing, detailed in this chapter, are well-established and routine procedures (e.g., DNA isolation, PCR and DNA sequencing) for most molecular biology laboratories, we highlight the analytic methods used to interpret the genotyping data generated by sequence analysis and their potential applications in local and global epidemiologic investigations.

Original languageEnglish (US)
Title of host publicationBacterial Pathogenesis - Methods and Protocols
Pages285-305
Number of pages21
StatePublished - Aug 31 2007

Publication series

NameMethods in Molecular Biology
Volume431
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • DNA sequencing
  • Epidemiology
  • Genotyping
  • Spa typing
  • Staphylococcus aureus
  • Variable number tandem repeats

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