Serum neutralizing antibody response to the vacuolating cytotoxin of Helicobacter pylori

T. L. Cover, P. Cao, U. K. Murthy, M. S. Sipple, Martin Blaser

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Approximately 50% of Helicobacter pylori isolates produce a cytotoxin in vitro that induces vacuolation of eukaryotic cells. To determine the in vivo relevance of this phenomenon, we sought to detect cytotoxin-neutralizing antibodies in sera from H. pylori-infected persons. As a group, sera from 29 H. pylori-infected patients neutralized the activity of the purified cytotoxin to a significantly greater extent than sera from 24 uninfected persons (P = 0.007). The cytotoxin neutralizing activity in sera from H. pylori-infected persons was mediated predominantly by the purified IgG fraction. Sera from H. pylori-infected persons neutralized the cytotoxins produced by multiple H. pylori strains, but failed to neutralize trimethylamine-induced cell vacuolation. Neutralization of cytotoxin activity by human or immune rabbit sera was associated with immunoblot IgG recognition of an 87-kD H. pylori protein. Similarly, neutralization of the toxin by sera was associated with IgG recognition of the purified cytotoxin in an enzyme- linked immunosorbent assay (P < 0.0001). The presence of cytotoxin- neutralizing antibodies in sera from H. pylori-infected persons indicates that the cytotoxin is synthesized in vivo.

Original languageEnglish (US)
Pages (from-to)913-918
Number of pages6
JournalJournal of Clinical Investigation
Volume90
Issue number3
DOIs
StatePublished - Jan 1 1992

Fingerprint

Cytotoxins
Neutralizing Antibodies
Helicobacter pylori
Antibody Formation
Serum
Immunoglobulin G
Eukaryotic Cells
Human Activities
Immune Sera
Enzyme-Linked Immunosorbent Assay
Rabbits

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Keywords

  • autophagy
  • gastritis
  • peptic ulcer
  • toxin
  • vacuole

Cite this

Cover, T. L. ; Cao, P. ; Murthy, U. K. ; Sipple, M. S. ; Blaser, Martin. / Serum neutralizing antibody response to the vacuolating cytotoxin of Helicobacter pylori. In: Journal of Clinical Investigation. 1992 ; Vol. 90, No. 3. pp. 913-918.
@article{13b555e2d26145748627066f3d5820b8,
title = "Serum neutralizing antibody response to the vacuolating cytotoxin of Helicobacter pylori",
abstract = "Approximately 50{\%} of Helicobacter pylori isolates produce a cytotoxin in vitro that induces vacuolation of eukaryotic cells. To determine the in vivo relevance of this phenomenon, we sought to detect cytotoxin-neutralizing antibodies in sera from H. pylori-infected persons. As a group, sera from 29 H. pylori-infected patients neutralized the activity of the purified cytotoxin to a significantly greater extent than sera from 24 uninfected persons (P = 0.007). The cytotoxin neutralizing activity in sera from H. pylori-infected persons was mediated predominantly by the purified IgG fraction. Sera from H. pylori-infected persons neutralized the cytotoxins produced by multiple H. pylori strains, but failed to neutralize trimethylamine-induced cell vacuolation. Neutralization of cytotoxin activity by human or immune rabbit sera was associated with immunoblot IgG recognition of an 87-kD H. pylori protein. Similarly, neutralization of the toxin by sera was associated with IgG recognition of the purified cytotoxin in an enzyme- linked immunosorbent assay (P < 0.0001). The presence of cytotoxin- neutralizing antibodies in sera from H. pylori-infected persons indicates that the cytotoxin is synthesized in vivo.",
keywords = "autophagy, gastritis, peptic ulcer, toxin, vacuole",
author = "Cover, {T. L.} and P. Cao and Murthy, {U. K.} and Sipple, {M. S.} and Martin Blaser",
year = "1992",
month = "1",
day = "1",
doi = "10.1172/JCI115967",
language = "English (US)",
volume = "90",
pages = "913--918",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "3",

}

Serum neutralizing antibody response to the vacuolating cytotoxin of Helicobacter pylori. / Cover, T. L.; Cao, P.; Murthy, U. K.; Sipple, M. S.; Blaser, Martin.

In: Journal of Clinical Investigation, Vol. 90, No. 3, 01.01.1992, p. 913-918.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Serum neutralizing antibody response to the vacuolating cytotoxin of Helicobacter pylori

AU - Cover, T. L.

AU - Cao, P.

AU - Murthy, U. K.

AU - Sipple, M. S.

AU - Blaser, Martin

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Approximately 50% of Helicobacter pylori isolates produce a cytotoxin in vitro that induces vacuolation of eukaryotic cells. To determine the in vivo relevance of this phenomenon, we sought to detect cytotoxin-neutralizing antibodies in sera from H. pylori-infected persons. As a group, sera from 29 H. pylori-infected patients neutralized the activity of the purified cytotoxin to a significantly greater extent than sera from 24 uninfected persons (P = 0.007). The cytotoxin neutralizing activity in sera from H. pylori-infected persons was mediated predominantly by the purified IgG fraction. Sera from H. pylori-infected persons neutralized the cytotoxins produced by multiple H. pylori strains, but failed to neutralize trimethylamine-induced cell vacuolation. Neutralization of cytotoxin activity by human or immune rabbit sera was associated with immunoblot IgG recognition of an 87-kD H. pylori protein. Similarly, neutralization of the toxin by sera was associated with IgG recognition of the purified cytotoxin in an enzyme- linked immunosorbent assay (P < 0.0001). The presence of cytotoxin- neutralizing antibodies in sera from H. pylori-infected persons indicates that the cytotoxin is synthesized in vivo.

AB - Approximately 50% of Helicobacter pylori isolates produce a cytotoxin in vitro that induces vacuolation of eukaryotic cells. To determine the in vivo relevance of this phenomenon, we sought to detect cytotoxin-neutralizing antibodies in sera from H. pylori-infected persons. As a group, sera from 29 H. pylori-infected patients neutralized the activity of the purified cytotoxin to a significantly greater extent than sera from 24 uninfected persons (P = 0.007). The cytotoxin neutralizing activity in sera from H. pylori-infected persons was mediated predominantly by the purified IgG fraction. Sera from H. pylori-infected persons neutralized the cytotoxins produced by multiple H. pylori strains, but failed to neutralize trimethylamine-induced cell vacuolation. Neutralization of cytotoxin activity by human or immune rabbit sera was associated with immunoblot IgG recognition of an 87-kD H. pylori protein. Similarly, neutralization of the toxin by sera was associated with IgG recognition of the purified cytotoxin in an enzyme- linked immunosorbent assay (P < 0.0001). The presence of cytotoxin- neutralizing antibodies in sera from H. pylori-infected persons indicates that the cytotoxin is synthesized in vivo.

KW - autophagy

KW - gastritis

KW - peptic ulcer

KW - toxin

KW - vacuole

UR - http://www.scopus.com/inward/record.url?scp=0026758223&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026758223&partnerID=8YFLogxK

U2 - 10.1172/JCI115967

DO - 10.1172/JCI115967

M3 - Article

VL - 90

SP - 913

EP - 918

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 3

ER -