Settling a family feud: a high-level phylogenomic framework for the Gentianales based on 353 nuclear genes and partial plastomes

Alexandre Antonelli, James J. Clarkson, Kent Kainulainen, Olivier Maurin, Grace E. Brewer, Aaron P. Davis, Niroshini Epitawalage, David J. Goyder, Tatyana Livshultz, Claes Persson, Lisa Pokorny, Shannon C.K. Straub, Lena Struwe, Alexandre R. Zuntini, Félix Forest, William J. Baker

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Premise: Comprising five families that vastly differ in species richness—ranging from Gelsemiaceae with 13 species to the Rubiaceae with 13,775 species—members of the Gentianales are often among the most species-rich and abundant plants in tropical forests. Despite considerable phylogenetic work within particular families and genera, several alternative topologies for family-level relationships within Gentianales have been presented in previous studies. Methods: Here we present a phylogenomic analysis based on nuclear genes targeted by the Angiosperms353 probe set for approximately 150 species, representing all families and approximately 85% of the formally recognized tribes. We were able to retrieve partial plastomes from off-target reads for most taxa and infer phylogenetic trees for comparison with the nuclear-derived trees. Results: We recovered high support for over 80% of all nodes. The plastid and nuclear data are largely in agreement, except for some weakly to moderately supported relationships. We discuss the implications of our results for the order’s classification, highlighting points of increased support for previously uncertain relationships. Rubiaceae is sister to a clade comprising (Gentianaceae + Gelsemiaceae) + (Apocynaceae + Loganiaceae). Conclusions: The higher-level phylogenetic relationships within Gentianales are confidently resolved. In contrast to recent studies, our results support the division of Rubiaceae into two subfamilies: Cinchonoideae and Rubioideae. We do not formally recognize Coptosapelteae and Luculieae within any particular subfamily but treat them as incertae sedis. Our framework paves the way for further work on the phylogenetics, biogeography, morphological evolution, and macroecology of this important group of flowering plants.

Original languageEnglish (US)
Pages (from-to)1143-1165
Number of pages23
JournalAmerican Journal of Botany
Volume108
Issue number7
DOIs
StatePublished - Jul 2021

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Plant Science

Keywords

  • Angiosperms353
  • asterids
  • Hyb-Seq
  • molecular phylogenetics
  • museomics
  • target sequence capture
  • tree of life

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