TY - JOUR
T1 - Sevoflurane Affects Oxidative Stress and Alters Apoptosis Status in Children and Cultured Neural Stem Cells
AU - Zhou, Xue
AU - Lu, Dihan
AU - Li, Wen da
AU - Chen, Xiao hui
AU - Yang, Xiao yu
AU - Chen, Xi
AU - Zhou, Zhi bin
AU - Ye, Jiang Hong
AU - Feng, Xia
N1 - Publisher Copyright:
© 2017, Springer Science+Business Media, LLC.
PY - 2018/5/1
Y1 - 2018/5/1
N2 - Anesthesia-induced neurotoxicity in immature animals has raised concerns about similar effects occurring in young children. Our study investigated two commonly used anesthetics—sevoflurane and propofol—for neurotoxicity in young children. Forty-seven children (aged 12–36 months) undergoing hypospadias repair surgery were randomized to receive sevoflurane (SG, n = 24) or propofol (PG, n = 23) general anesthesia. Venous blood was collected at three different times—immediately after induction, 2 h, and 3 days after surgery. The cellular portion was assessed for antioxidant defense and DNA damage, using enzyme assay kits and qRT-PCR, respectively, while serum was used to treat cultured neural stem cells (NSCs). MTT assay and TUNEL staining were performed, and the mRNA levels of antioxidant enzymes and apoptosis indicators were evaluated by qRT-PCR. Antioxidant defense and apoptosis status in the SG group were significantly higher than in the PG group at 2 h after surgery. Additionally, exposure of NSCs to postoperative serum of the SG group resulted in decreased cell density and viability, increased TUNEL-positive cells, elevated mRNA levels of antioxidant enzymes, and cleaved caspase-3 expression. Our data shows for the first time that in young children, administration of sevoflurane, but not propofol, leads to temporally increased antioxidant defense and apoptosis status as well as damage of NSCs.
AB - Anesthesia-induced neurotoxicity in immature animals has raised concerns about similar effects occurring in young children. Our study investigated two commonly used anesthetics—sevoflurane and propofol—for neurotoxicity in young children. Forty-seven children (aged 12–36 months) undergoing hypospadias repair surgery were randomized to receive sevoflurane (SG, n = 24) or propofol (PG, n = 23) general anesthesia. Venous blood was collected at three different times—immediately after induction, 2 h, and 3 days after surgery. The cellular portion was assessed for antioxidant defense and DNA damage, using enzyme assay kits and qRT-PCR, respectively, while serum was used to treat cultured neural stem cells (NSCs). MTT assay and TUNEL staining were performed, and the mRNA levels of antioxidant enzymes and apoptosis indicators were evaluated by qRT-PCR. Antioxidant defense and apoptosis status in the SG group were significantly higher than in the PG group at 2 h after surgery. Additionally, exposure of NSCs to postoperative serum of the SG group resulted in decreased cell density and viability, increased TUNEL-positive cells, elevated mRNA levels of antioxidant enzymes, and cleaved caspase-3 expression. Our data shows for the first time that in young children, administration of sevoflurane, but not propofol, leads to temporally increased antioxidant defense and apoptosis status as well as damage of NSCs.
KW - Anesthetics
KW - Apoptosis
KW - Developing brain
KW - Neural stem cells
KW - Oxidative stress
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U2 - 10.1007/s12640-017-9827-5
DO - 10.1007/s12640-017-9827-5
M3 - Article
C2 - 29071560
AN - SCOPUS:85032180361
SN - 1029-8428
VL - 33
SP - 790
EP - 800
JO - Neurotoxicity Research
JF - Neurotoxicity Research
IS - 4
ER -