TY - JOUR
T1 - Shape changes of erythrocytes during blood clot contraction and the structure of polyhedrocytes
AU - Tutwiler, Valerie
AU - Mukhitov, Alexander R.
AU - Peshkova, Alina D.
AU - Le Minh, Giang
AU - Khismatullin, R. R.
AU - Vicksman, Jacqueline
AU - Nagaswami, Chandrasekaran
AU - Litvinov, Rustem I.
AU - Weisel, John W.
N1 - Funding Information:
The work was supported by NIH grants UO1HL116330, and T32 H10791, National Science Foundation grant DMR1505662, the Program for Competitive Growth at Kazan Federal University, grant 18-415-160004 of the Russian Foundation for Basic Research and Republic of Tatarstan, American Heart Association grant 16PRE30260002, and a grant from Boston Scientific The authors thank vascular surgeons Drs Dmitry V.
Publisher Copyright:
© 2018, The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Polyhedral erythrocytes, named polyhedrocytes, are formed in contracted blood clots and thrombi, as a result of compression by activated contractile platelets pulling on fibrin. This deformation was shown to be mechanical in nature and polyhedrocytes were characterized using light and electron microscopy. Through three-dimensional reconstruction, we quantified the geometry of biconcave, intermediate, and polyhedral erythrocytes within contracting blood clots. During compression, erythrocytes became less oblate and more prolate than the biconcave cells and largely corresponded to convex, irregular polyhedra with a total number of faces ranging from 10 to 16. Faces were polygons with 3 to 6 sides. The majority of the faces were quadrilaterals, though not all sides were straight and not all faces were flat. There were no changes in the surface area or volume. These results describe the gradual natural deformation of erythrocytes as a part of compaction into a tightly packed array that is an important but understudied component of mature blood clots and thrombi.
AB - Polyhedral erythrocytes, named polyhedrocytes, are formed in contracted blood clots and thrombi, as a result of compression by activated contractile platelets pulling on fibrin. This deformation was shown to be mechanical in nature and polyhedrocytes were characterized using light and electron microscopy. Through three-dimensional reconstruction, we quantified the geometry of biconcave, intermediate, and polyhedral erythrocytes within contracting blood clots. During compression, erythrocytes became less oblate and more prolate than the biconcave cells and largely corresponded to convex, irregular polyhedra with a total number of faces ranging from 10 to 16. Faces were polygons with 3 to 6 sides. The majority of the faces were quadrilaterals, though not all sides were straight and not all faces were flat. There were no changes in the surface area or volume. These results describe the gradual natural deformation of erythrocytes as a part of compaction into a tightly packed array that is an important but understudied component of mature blood clots and thrombi.
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U2 - 10.1038/s41598-018-35849-8
DO - 10.1038/s41598-018-35849-8
M3 - Article
C2 - 30559364
AN - SCOPUS:85058724785
SN - 2045-2322
VL - 8
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 17907
ER -