Simple freeze fracture technique for scanning election microscopy of collagenous biomaterials

David Christiansen, George Pins, Frederick H. Silver

Research output: Contribution to journalArticlepeer-review

Abstract

A simple freeze fracture technique for the ultra-structural analysis of fragile collagenous biomaterials is presented. Following standard methods of preparation and dehydration, fragile biomaterials are fractured with a sharp blade attached to a modified slide hammer. The sliding mass imparts a sudden impact load on the frozen surface of the material, creating a distortion free cleavage plane across the material. More traditional methods including bending and cutting with scissors introduce artifacts indirectly associated with stress concentration produced by bending, and sample compression at the edges with cutting. The impact leading of the sample during fracture results in preservation of edge detail, showing clear structural features up to the surface of the collagenous biomaterial.

Original languageEnglish (US)
Pages (from-to)189-191
Number of pages3
JournalCells and Materials
Volume3
Issue number2
StatePublished - Dec 1 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Biophysics

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