Simple high‐pressure liquid chromatographic determination of trisulfapyrimidines in human serum

Thomas J. Goehl, Lalit K. Mathur, Jeffrey D. Strum, James M. Jaffe, William H. Pitlick, Vinod P. Shah, Rolland I. Poust, John L. Colaizzi

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

A simple and rapid high‐pressure liquid chromatographic method was developed for the determination of sulfadiazine, sulfamerazine, and sulfamethazine in human serum. After the trichloroacetic acid precipitation of the serum proteins, an aliquot of the supernate is injected into a high‐pressure liquid chromatograph equipped with a reversed‐phase microparticulate column and a fixed wavelength UV detector. For each of the three components of trisulfapyrimidines, a linear calibration curve was observed in the 1–30‐μg/ml range, with the precision of the assay estimated to be ±2% (RSD). Preliminary pharmacokinetic data are also presented.

Original languageEnglish (US)
Pages (from-to)404-406
Number of pages3
JournalJournal of Pharmaceutical Sciences
Volume67
Issue number3
DOIs
StatePublished - Mar 1978
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Keywords

  • Antibacterials—sulfadiazine, sulfamethazine, and sulfamerazine, high‐pressure liquid chromatographic analyses in human serum
  • High‐pressure liquid chromatography—analyses, sulfadiazine, sulfamethazine, and sulfamerazine in human serum
  • Sulfadiazine—high‐pressure liquid chromatographic analysis in human serum
  • Sulfamerazine—high‐pressure liquid chromatographic analysis in human serum
  • Sulfamethazine—high‐pressure liquid chromatographic analysis in human serum
  • Trisulfapyrimidines—high‐pressure liquid chromatographic analyses in human serum

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