Single-stranded DNA genome in a whitefly-transmitted plant virus

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Bean golden yellow mosaic virus (GYMV) DNA sedimented in sucrose density gradients and in an analytical ultracentrifuge as a single component. Values of s20,w which depended upon ionic strength (i) and pH, were 16.0 (pH = 7.0, i = 0.1); 16.3 (pH = 7.0, i = 1.0); 9.0 (pH = 13, i = 0.1); and 12.0 (0.1 N NaOH, i = 1.0). GYMV-DNA banded in CsCl as a single component with a buoyant density of 1.7170 g/ml and exhibited hyperchromicity when heated between 20 and 70°. Hyperchromicity and a shift in the ultraviolet light absorption maximum were observed when the DNA was treated with formaldehyde (1.8%) at room temperature. Nuclease sensitivity tests showed that the DNA was susceptible to hydrolysis by DNase I and nuclease S1 (specific for single-stranded DNA) but not to hydrolysis by RNase A or by 0.3 N NaOH. Molecular weight values calculated from sedimentation velocity and CsCl equilibrium data were in the range of 0.66 to 0.95 × 106 daltons GYMV thus contains DNA with the properties of a predominantly single-stranded molecule and represents the first reported single-stranded DNA virus from plants.

Original languageEnglish (US)
Pages (from-to)171-179
Number of pages9
Issue number1
StatePublished - Nov 1977
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Virology


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