TY - JOUR
T1 - Single-stranded DNA genome in a whitefly-transmitted plant virus
AU - Goodman, Robert M.
N1 - Funding Information:
I thank Pornpod Thongmeearkom and Teresa L. Shock for capable technical assistance and Dr. N. S. Slavik for the Micrococcus Zuteus DNA. Research was supported by the U.S. Agency for International Development Research Contract AID TA/C-1294 to the International Soybean Program at the University of Illinois and by the Illinois Agricultural Experiment Station. I extend special gratitude to Dr. Julio Bird who kindly provided the original inoculum and information from recent studies as yet not published.
PY - 1977/11
Y1 - 1977/11
N2 - Bean golden yellow mosaic virus (GYMV) DNA sedimented in sucrose density gradients and in an analytical ultracentrifuge as a single component. Values of s20,w which depended upon ionic strength (i) and pH, were 16.0 (pH = 7.0, i = 0.1); 16.3 (pH = 7.0, i = 1.0); 9.0 (pH = 13, i = 0.1); and 12.0 (0.1 N NaOH, i = 1.0). GYMV-DNA banded in CsCl as a single component with a buoyant density of 1.7170 g/ml and exhibited hyperchromicity when heated between 20 and 70°. Hyperchromicity and a shift in the ultraviolet light absorption maximum were observed when the DNA was treated with formaldehyde (1.8%) at room temperature. Nuclease sensitivity tests showed that the DNA was susceptible to hydrolysis by DNase I and nuclease S1 (specific for single-stranded DNA) but not to hydrolysis by RNase A or by 0.3 N NaOH. Molecular weight values calculated from sedimentation velocity and CsCl equilibrium data were in the range of 0.66 to 0.95 × 106 daltons GYMV thus contains DNA with the properties of a predominantly single-stranded molecule and represents the first reported single-stranded DNA virus from plants.
AB - Bean golden yellow mosaic virus (GYMV) DNA sedimented in sucrose density gradients and in an analytical ultracentrifuge as a single component. Values of s20,w which depended upon ionic strength (i) and pH, were 16.0 (pH = 7.0, i = 0.1); 16.3 (pH = 7.0, i = 1.0); 9.0 (pH = 13, i = 0.1); and 12.0 (0.1 N NaOH, i = 1.0). GYMV-DNA banded in CsCl as a single component with a buoyant density of 1.7170 g/ml and exhibited hyperchromicity when heated between 20 and 70°. Hyperchromicity and a shift in the ultraviolet light absorption maximum were observed when the DNA was treated with formaldehyde (1.8%) at room temperature. Nuclease sensitivity tests showed that the DNA was susceptible to hydrolysis by DNase I and nuclease S1 (specific for single-stranded DNA) but not to hydrolysis by RNase A or by 0.3 N NaOH. Molecular weight values calculated from sedimentation velocity and CsCl equilibrium data were in the range of 0.66 to 0.95 × 106 daltons GYMV thus contains DNA with the properties of a predominantly single-stranded molecule and represents the first reported single-stranded DNA virus from plants.
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U2 - 10.1016/0042-6822(77)90220-3
DO - 10.1016/0042-6822(77)90220-3
M3 - Article
C2 - 18625485
AN - SCOPUS:0017731289
SN - 0042-6822
VL - 83
SP - 171
EP - 179
JO - Virology
JF - Virology
IS - 1
ER -