@article{fee7cd368427434f9d3da74703f25d92,
title = "SIRT1 stabilizes PML promoting its sumoylation",
abstract = "SIRT1, the closest mammalian homolog of yeast Sir2, is an NAD-dependent deacetylase with relevant functions in cancer, aging, and metabolism among other processes. SIRT1 has a diffuse nuclear localization but is recruited to the PML nuclear bodies (PML-NBs) after PML upregulation. However, the functions of SIRT1 in the PML-NBs are unknown. In this study we show that primary mouse embryo fibroblasts lacking SIRT1 contain reduced PML protein levels that are increased after reintroduction of SIRT1. In addition, overexpression of SIRT1 in HEK-293 cells increases the amount of PML protein whereas knockdown of SIRT1 reduces the size and number of PML-NBs and the levels of PML protein in HeLa cells. SIRT1 stimulates PML sumoylation in vitro and in vivo in a deacetylase-independent manner. Importantly, the absence of SIRT1 reduces the apoptotic response of vesicular stomatitis virus-infected cells and favors the extent of this PML-sensitive virus replication. These results show a novel function of SIRT1 in the control of PML and PML-NBs.",
keywords = "PML, SIRT1, SUMO, VSV, antiviral activity",
author = "M. Campagna and D. Herranz and Garcia, {M. A.} and L. Marcos-Villar and J. Gonz{\'a}lez-Santamar{\'i}a and P. Gallego and S. Gutierrez and M. Collado and M. Serrano and M. Esteban and C. Rivas",
note = "Funding Information: Acknowledgements. We acknowledge the technical assistance of Victoria Jim{\'e}nez and Maribel Mu{\~n}oz. We are indebted to Dr. Michael Greenberg (Children{\textquoteright}s Hospital Center for Life Sciences), Dr. Kun-Sang Chang (MD Anderson Cancer Center, Houston), Dr. Tony Kouzarides (Gurdon Institute, University of Cambridge), Dr. Toren Finkel (National Heart Lung and Blood Institute), and Dr. Keith D Robertson (Shands Cancer Center, University of Florida), for kindly providing reagents. Funding at the laboratory of CR is provided by BFU-2008-03784 and CSIC. ME is supported by SAF2008-02036 and Foundation Bot{\'i}n. M Campagna is supported by Fundaci{\'o}n Bot{\'i}n and Juan de la Cierva Programme. D Herranz is supported by a predoctoral fellowship from the Spanish Ministry of Health and from the {\textquoteleft}Francisco Cobos{\textquoteright} Foundation. M Collado is an investigator of the Ram{\'o}n y Cajal Programme. The funders had no role in the study design, data collection and analysis, and decision to publish, or in the preparation of the paper.",
year = "2011",
month = jan,
doi = "10.1038/cdd.2010.77",
language = "English (US)",
volume = "18",
pages = "72--79",
journal = "Cell Death and Differentiation",
issn = "1350-9047",
publisher = "Nature Publishing Group",
number = "1",
}