Global investigation of poly(A) tails has been hindered by technical challenges. In a recent advance, two groups developed deep sequencing methods to globally interrogate poly(A) tail length and sequence with high precision, opening new avenues for investigation of poly(A) tail functions in mRNA metabolism. Initial applications of these methods reveal insights into the relationship between poly(A) tail length and translational efficiency, and identify widespread uridylation and guanylation at the 3' ends of transcripts.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- MRNA stability
- Poly(A) tail
- Post-transcriptional control