Smart surfaces: Use of electrokinetics for selective modulation of biomolecular affinities

Sam Emaminejad, Mehdi Javanmard, Robert W. Dutton, Ronald W. Davis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

With the aid of negative dielectrophoresis (nDEP) force in conjunction with shear force and at an optimal sodium hydroxide (NaOH) concentration we demonstrated a switch-like functionality to elute immuno-bound beads from the surface. At an optimal flow rate and NaOH concentration, nDEP turned on results in bead detachment, whereas when nDEP is off, the beads remain attached. This platform offers the potential for performing a bead-based multiplexed immunoassay where in a single channel various regions are immobilized with a different antibody, each targeting a different antigen. As a proof of concept we demonstrated the ability of nDEP to provide this switching behavior in a singleplex assay for the interactions that were in the same order of magnitude in strength as typical antibody-antigen interactions.

Original languageEnglish (US)
Title of host publicationMEMS, BioMEMS and Bioelectronics - Materials and Devices
Pages153-158
Number of pages6
DOIs
StatePublished - 2012
Externally publishedYes
Event2011 MRS Fall Meeting - Boston, MA, United States
Duration: Nov 28 2011Dec 2 2011

Publication series

NameMaterials Research Society Symposium Proceedings
Volume1415
ISSN (Print)0272-9172

Other

Other2011 MRS Fall Meeting
Country/TerritoryUnited States
CityBoston, MA
Period11/28/1112/2/11

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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