Soft polymer-based cantilever probe for AFM nanoindentation of live mammalian cells in liquid

F. Yu, J. Liu, S. Yu, Z. Yang, Y. Pan, N. Gao, Q. Zou, J. Jeon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

A soft Poly(Methyl-Meth-Acrylate)(PMMA)-polymerbased V-shaped cantilever probe is proposed for atomic force microscopy (AFM), and the first well-functioning, visually-flat prototype fabricated using a simple, but novel low-cost process and bio-compatible materials is demonstrated. With the prototype, for the first time, AFM nanoindentation was performed on live mammalian cells - human cervical adenocarcinoma cells in liquid. Results obtained using the prototype were compared to those obtained using a commercial Si3N4 cantilever probe. Minimal or no versus severe deformation and/or distortion of the cell membrane was observed when using the prototype versus the Si3N4 probe.

Original languageEnglish (US)
Title of host publication2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages2260-2263
Number of pages4
ISBN (Electronic)9781479989553
DOIs
StatePublished - Aug 5 2015
Event18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States
Duration: Jun 21 2015Jun 25 2015

Publication series

Name2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

Other

Other18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
CountryUnited States
CityAnchorage
Period6/21/156/25/15

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Electrical and Electronic Engineering

Keywords

  • AFM
  • Live Cells
  • Nanoindentation
  • Nanomechanical Measurements
  • PMMA
  • Polymer Cantilever Probes

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