Soluble Factors from Liver and Hepatomas Which Inhibit [3 H]Thymidine Incorporation into DNA of Novikoff Hepatoma Cells 1

Rosemary Barra, Janey Parsons, Michael R. Koch, Michael A. Lea

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10 Scopus citations

Abstract

The nature of soluble factors from liver and hepatomas which inhibit [3 H]thymidine incorporation into DNA was studied in Novikoff hepatoma cells. The decreased activity in hepatoma preparations was due to loss of a high-molecular-weight heat-labile factor. Although this factor cochromatographed with arginase activity on Sephadex G-150, it does not appear to result from this activity as judged by the failure of arginine to prevent the inhibitory effect on [3 H]thymidine incorporation. Both liver and hepatomas contained a heat-stable factor with inhibitory activity. Studies with ethanol-soluble material suggested that the action was not solely attributable to the presence of unlabeled thymidine, since the apparent molecular weight was too high and since the factors) inhibited [3 H]leucine incorporation into protein in addition to inhibiting [3 H]thymidine incorporation in DNA.

Original languageEnglish (US)
Pages (from-to)1655-1660
Number of pages6
JournalCancer Research
Volume39
Issue number5
StatePublished - May 1 1979

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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