Solution NMR and X-ray crystal structures of membrane-associated Lipoprotein-17 domain reveal a novel fold

Rajeswari Mani, Sergey Vorobiev, G. V.T. Swapna, Helen Neely, Haleema Janjua, Colleen Ciccosanti, Rong Xiao, Thomas B. Acton, John K. Everett, John Hunt, Gaetano T. Montelione

Research output: Contribution to journalArticlepeer-review


The conserved Lipoprotein-17 domain of membrane-associated protein Q9PRA0-UREPA from Ureaplasma parvum was selected for structure determination by the Northeast Structural Genomics Consortium, as part of the Protein Structure Initiative's program on structure-function analysis of protein domains from large domain sequence families lacking structural representatives. The 100-residue Lipoprotein-17 domain is a "domain of unknown function" (DUF) that is a member of Pfam protein family PF04200, a large domain family for which no members have characterized biochemical functions. The three-dimensional structure of the Lipoprotein-17 domain of protein Q9PRA0-UREPA was determined by both solution NMR and by X-ray crystallography at 2.5 Å. The two structures are in good agreement with each other. The domain structure features three α-helices, α1 through α3, and five β-strands. Strands β1/β2, β3/β4, β4/β5 are anti-parallel to each other. Strands β1and β2 are orthogonal to strands β3, β4, β5, while helix α3 is formed between the strands β3 and β4. One-turn helix α2 is formed between the strands β1 and β2, while helix α1 occurs in the N-terminal polypeptide segment. Searches of the Protein Data Bank do not identify any other protein with significant structural similarity to Lipoprotein-17 domain of Q9PRA0-UREPA, indicating that it is a novel protein fold.

Original languageEnglish (US)
Pages (from-to)27-32
Number of pages6
JournalJournal of Structural and Functional Genomics
Issue number1
StatePublished - Mar 2011

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Genetics


  • Domains of unknown function (DUFs)
  • Lipoprotein
  • Lipoprotein-17 domain of Q9PRA0-UREPA
  • Northeast Structural Genomics Consortium (NESG)
  • Solution NMR
  • Structural genomics
  • Ureaplasma parvum
  • X-ray crystal structure


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