Solution NMR and X-ray crystal structures of Pseudomonas syringae Pspto-3016 from protein domain family PF04237 (DUF419) adopt a "double wing" DNA binding motif

Erik A. Feldmann; Jayaraman Seetharaman; Theresa A. Ramelot; Scott Lew; Li Zhao; Keith Hamilton; Colleen Ciccosanti; Rong Xiao; Thomas B. Acton; John K. Everett; Liang Tong; Gaetano T. Montelione; Michael A. Kennedy

doi:10.1007/s10969-012-9140-8

Solution NMR and X-ray crystal structures of Pseudomonas syringae Pspto-3016 from protein domain family PF04237 (DUF419) adopt a "double wing" DNA binding motif

Erik A. Feldmann, Jayaraman Seetharaman, Theresa A. Ramelot, Scott Lew, Li Zhao, Keith Hamilton, Colleen Ciccosanti, Rong Xiao, Thomas B. Acton, John K. Everett, Liang Tong, Gaetano T. Montelione, Michael A. Kennedy

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The protein Pspto-3016 is a 117-residue member of the protein domain family PF04237 (DUF419), which is to date a functionally uncharacterized family of proteins. In this report, we describe the structure of Pspto-3016 from Pseudomonas syringae solved by both solution NMR and X-ray crystallography at 2.5 Å resolution. In both cases, the structure of Pspto-3016 adopts a "double wing" α/β sandwich fold similar to that of protein YjbR from Escherichia coli and to the C-terminal DNA binding domain of the MotA transcription factor (MotCF) from T4 bacteriophage, along with other uncharacterized proteins. Pspto-3016 was selected by the Protein Structure Initiative of the National Institutes of Health and the Northeast Structural Genomics Consortium (NESG ID PsR293).

Original language	English (US)
Pages (from-to)	155-162
Number of pages	8
Journal	Journal of Structural and Functional Genomics
Volume	13
Issue number	3
DOIs	https://doi.org/10.1007/s10969-012-9140-8
State	Published - Sep 2012

All Science Journal Classification (ASJC) codes

Structural Biology
Biochemistry
Genetics

Keywords

2KFP
3H9X
DUF419
Double wing
NMR
PF04237
Pspto-3016
Structural genomics
X-ray crystallography

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Feldmann, E. A., Seetharaman, J., Ramelot, T. A., Lew, S., Zhao, L., Hamilton, K., Ciccosanti, C., Xiao, R., Acton, T. B., Everett, J. K., Tong, L., Montelione, G. T., & Kennedy, M. A. (2012). Solution NMR and X-ray crystal structures of Pseudomonas syringae Pspto-3016 from protein domain family PF04237 (DUF419) adopt a "double wing" DNA binding motif. Journal of Structural and Functional Genomics, 13(3), 155-162. https://doi.org/10.1007/s10969-012-9140-8

Feldmann, Erik A. ; Seetharaman, Jayaraman ; Ramelot, Theresa A. ; Lew, Scott ; Zhao, Li ; Hamilton, Keith ; Ciccosanti, Colleen ; Xiao, Rong ; Acton, Thomas B. ; Everett, John K. ; Tong, Liang ; Montelione, Gaetano T. ; Kennedy, Michael A. / Solution NMR and X-ray crystal structures of Pseudomonas syringae Pspto-3016 from protein domain family PF04237 (DUF419) adopt a "double wing" DNA binding motif. In: Journal of Structural and Functional Genomics. 2012 ; Vol. 13, No. 3. pp. 155-162.

@article{b80b63fde0aa40dba75a58c1f0a1c314,

title = "Solution NMR and X-ray crystal structures of Pseudomonas syringae Pspto-3016 from protein domain family PF04237 (DUF419) adopt a {"}double wing{"} DNA binding motif",

abstract = "The protein Pspto-3016 is a 117-residue member of the protein domain family PF04237 (DUF419), which is to date a functionally uncharacterized family of proteins. In this report, we describe the structure of Pspto-3016 from Pseudomonas syringae solved by both solution NMR and X-ray crystallography at 2.5 {\AA} resolution. In both cases, the structure of Pspto-3016 adopts a {"}double wing{"} α/β sandwich fold similar to that of protein YjbR from Escherichia coli and to the C-terminal DNA binding domain of the MotA transcription factor (MotCF) from T4 bacteriophage, along with other uncharacterized proteins. Pspto-3016 was selected by the Protein Structure Initiative of the National Institutes of Health and the Northeast Structural Genomics Consortium (NESG ID PsR293).",

keywords = "2KFP, 3H9X, DUF419, Double wing, NMR, PF04237, Pspto-3016, Structural genomics, X-ray crystallography",

author = "Feldmann, {Erik A.} and Jayaraman Seetharaman and Ramelot, {Theresa A.} and Scott Lew and Li Zhao and Keith Hamilton and Colleen Ciccosanti and Rong Xiao and Acton, {Thomas B.} and Everett, {John K.} and Liang Tong and Montelione, {Gaetano T.} and Kennedy, {Michael A.}",

note = "Funding Information: Acknowledgments The authors thank John A. Schwanof and Randy Abramowitz for their support at the X4 beam line, National Synchrotron Light Source, Brookhaven National Lab. This work was supported by grants from the National Institute of General Medical Sciences Protein Structure Initiative (PSI) of the National Institutes of Health, PSI-2 (P50 GM 074958) and PSI:Biology (U54-GM094597). NMR data collection was conducted at the Ohio Center of Excellence in Biomedicine in Structural Biology and Metabonomics at Miami University.",

year = "2012",

month = sep,

doi = "10.1007/s10969-012-9140-8",

language = "English (US)",

volume = "13",

pages = "155--162",

journal = "Journal of Structural and Functional Genomics",

issn = "1345-711X",

publisher = "Springer Netherlands",

number = "3",

}

Feldmann, EA, Seetharaman, J, Ramelot, TA, Lew, S, Zhao, L, Hamilton, K, Ciccosanti, C, Xiao, R, Acton, TB, Everett, JK, Tong, L, Montelione, GT & Kennedy, MA 2012, 'Solution NMR and X-ray crystal structures of Pseudomonas syringae Pspto-3016 from protein domain family PF04237 (DUF419) adopt a "double wing" DNA binding motif', Journal of Structural and Functional Genomics, vol. 13, no. 3, pp. 155-162. https://doi.org/10.1007/s10969-012-9140-8

Solution NMR and X-ray crystal structures of Pseudomonas syringae Pspto-3016 from protein domain family PF04237 (DUF419) adopt a "double wing" DNA binding motif. / Feldmann, Erik A.; Seetharaman, Jayaraman; Ramelot, Theresa A.; Lew, Scott; Zhao, Li; Hamilton, Keith; Ciccosanti, Colleen; Xiao, Rong; Acton, Thomas B.; Everett, John K.; Tong, Liang; Montelione, Gaetano T.; Kennedy, Michael A.

In: Journal of Structural and Functional Genomics, Vol. 13, No. 3, 09.2012, p. 155-162.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Solution NMR and X-ray crystal structures of Pseudomonas syringae Pspto-3016 from protein domain family PF04237 (DUF419) adopt a "double wing" DNA binding motif

AU - Feldmann, Erik A.

AU - Seetharaman, Jayaraman

AU - Ramelot, Theresa A.

AU - Lew, Scott

AU - Zhao, Li

AU - Hamilton, Keith

AU - Ciccosanti, Colleen

AU - Xiao, Rong

AU - Acton, Thomas B.

AU - Everett, John K.

AU - Tong, Liang

AU - Montelione, Gaetano T.

AU - Kennedy, Michael A.

N1 - Funding Information: Acknowledgments The authors thank John A. Schwanof and Randy Abramowitz for their support at the X4 beam line, National Synchrotron Light Source, Brookhaven National Lab. This work was supported by grants from the National Institute of General Medical Sciences Protein Structure Initiative (PSI) of the National Institutes of Health, PSI-2 (P50 GM 074958) and PSI:Biology (U54-GM094597). NMR data collection was conducted at the Ohio Center of Excellence in Biomedicine in Structural Biology and Metabonomics at Miami University.

PY - 2012/9

Y1 - 2012/9

N2 - The protein Pspto-3016 is a 117-residue member of the protein domain family PF04237 (DUF419), which is to date a functionally uncharacterized family of proteins. In this report, we describe the structure of Pspto-3016 from Pseudomonas syringae solved by both solution NMR and X-ray crystallography at 2.5 Å resolution. In both cases, the structure of Pspto-3016 adopts a "double wing" α/β sandwich fold similar to that of protein YjbR from Escherichia coli and to the C-terminal DNA binding domain of the MotA transcription factor (MotCF) from T4 bacteriophage, along with other uncharacterized proteins. Pspto-3016 was selected by the Protein Structure Initiative of the National Institutes of Health and the Northeast Structural Genomics Consortium (NESG ID PsR293).

AB - The protein Pspto-3016 is a 117-residue member of the protein domain family PF04237 (DUF419), which is to date a functionally uncharacterized family of proteins. In this report, we describe the structure of Pspto-3016 from Pseudomonas syringae solved by both solution NMR and X-ray crystallography at 2.5 Å resolution. In both cases, the structure of Pspto-3016 adopts a "double wing" α/β sandwich fold similar to that of protein YjbR from Escherichia coli and to the C-terminal DNA binding domain of the MotA transcription factor (MotCF) from T4 bacteriophage, along with other uncharacterized proteins. Pspto-3016 was selected by the Protein Structure Initiative of the National Institutes of Health and the Northeast Structural Genomics Consortium (NESG ID PsR293).

KW - 2KFP

KW - 3H9X

KW - DUF419

KW - Double wing

KW - NMR

KW - PF04237

KW - Pspto-3016

KW - Structural genomics

KW - X-ray crystallography

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U2 - 10.1007/s10969-012-9140-8

DO - 10.1007/s10969-012-9140-8

M3 - Article

C2 - 22865330

AN - SCOPUS:84865467773

VL - 13

SP - 155

EP - 162

JO - Journal of Structural and Functional Genomics

JF - Journal of Structural and Functional Genomics

SN - 1345-711X

IS - 3

ER -