Solution NMR structure of the iron-sulfur cluster assembly protein U (IscU) with zinc bound at the active site

Theresa A. Ramelot, John R. Cort, Sharon Goldsmith-Fischman, Gregory J. Kornhaber, Rong Xiao, Ritu Shastry, Thomas B. Acton, Barry Honig, Gaetano T. Montelione, Michael A. Kennedy

Research output: Contribution to journalArticlepeer-review

112 Scopus citations

Abstract

IscU is a highly conserved protein that serves as the scaffold for IscS-mediated assembly of iron-sulfur ([Fe-S]) clusters. We report the NMR solution structure of monomeric Haemophilus influenzae IscU with zinc bound at the [Fe-S] cluster assembly site. The compact core of the globular structure has an α-β sandwich architecture with a three-stranded antiparallel β-sheet and four α-helices. A nascent helix is located N-terminal to the core structure. The zinc is ligated by three cysteine residues and one histidine residue that are located in and near conformationally dynamic loops at one end of the IscU structure. Removal of the zinc metal by chelation results in widespread loss of structure in the apo form. The zinc-bound IscU may be a good model for iron-loaded IscU and may demonstrate structural features found in the [Fe-S] cluster bound form. Structural and functional similarities, genomic context in operons containing other homologous genes, and distributions of conserved surface residues support the hypothesis that IscU protein domains are homologous (i.e. derived from a common ancestor) with the SufE/YgdK family of [Fe-S] cluster assembly proteins.

Original languageEnglish (US)
Pages (from-to)567-583
Number of pages17
JournalJournal of molecular biology
Volume344
Issue number2
DOIs
StatePublished - Nov 19 2004

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Keywords

  • IscU
  • NMR structure
  • Zn-binding
  • iron-sulfur clusters
  • zinc

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