Somatic cell mapping of the bovine interferon-α receptor

Jerome A. Langer, Rengarajulu Puvanakrishnan, James E. Womack

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The bovine interferon-α receptor (BoIFN-αR) mediates the activity of bovine IFN-αs and IFN-β. In addition, human IFN-αs have uniformly high biological activity on bovine cells. A 32P-labeled derivative of human recombinant IFN-αA (HuIFN-αA-P1) binds well and can form a characteristic 130-kDa complex on bovine cells, but not on hamster cells. We have, therefore, analyzed the binding and covalent crosslinking of [32P]HuIFN-αA-P1 to a panel of bovine-hamster somatic cell hybrids. Binding to several bovine-hamster hybrid cell lines was strong (about 30-50% of that seen with bovine MDBK cells) and specific. The binding correlated uniquely with bovine syntenic group U10. In several of the hybrid lines, the ability of human IFN-αB to enhance the expression of endogenous MHC class I molecules correlated with the binding results. We thus conclude that the bovine IFN-αR structural gene (locus designation IFNAR) localizes to syntenic group U10. This group includes a number of other genes whose homologs map to human Chromosome (Chr) 21.

Original languageEnglish (US)
Pages (from-to)237-240
Number of pages4
JournalMammalian Genome
Issue number4
StatePublished - Apr 1992

All Science Journal Classification (ASJC) codes

  • Genetics


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