Somatic embryogenesis from immature inflorescences of oil palm

J. B. Teixeira, M. R. Söndahl, Edward Kirby

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Immature inflorescences of oil palm (Elaeis guineensis) var. Pisifera were inoculated onto modified MS medium containing 0.3% (w/v) activated charcoal and 475 μM 2,4-D. After 2-3 months of culture, a hard yellow callus proliferated at the base of the shoot-like structures. The high incidence of phenolic oxidation required the use of increased levels of activated charcoal (0.5% w/v) and 2,4-D (500 μM). Development of floral structures from inflorescence expiants was frequently observed during the culture period. After 81 weeks of culture, an embryogenic tissue characterized by compact consistency and pearly white color was observed in tissues derived from very young inflorescences. This compact embryogenic tissue differentiated into normal somatic embryos when transferred onto regeneration medium containing NAA (15 μM) and ABA (2 μM). Normal plantlets were recovered from these somatic embryos after 8 weeks on regeneration medium.

Original languageEnglish (US)
Pages (from-to)247-250
Number of pages4
JournalPlant Cell Reports
Volume13
Issue number5
DOIs
StatePublished - Feb 1 1994

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Elaeis guineensis
somatic embryogenesis
inflorescences
immatures
activated carbon
somatic embryos
2,4-D
angle of incidence
tissue culture
plantlets
callus
oxidation
flowering
shoots
color
tissues

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Teixeira, J. B. ; Söndahl, M. R. ; Kirby, Edward. / Somatic embryogenesis from immature inflorescences of oil palm. In: Plant Cell Reports. 1994 ; Vol. 13, No. 5. pp. 247-250.
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Teixeira, JB, Söndahl, MR & Kirby, E 1994, 'Somatic embryogenesis from immature inflorescences of oil palm', Plant Cell Reports, vol. 13, no. 5, pp. 247-250. https://doi.org/10.1007/BF00233313

Somatic embryogenesis from immature inflorescences of oil palm. / Teixeira, J. B.; Söndahl, M. R.; Kirby, Edward.

In: Plant Cell Reports, Vol. 13, No. 5, 01.02.1994, p. 247-250.

Research output: Contribution to journalArticle

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