Somatic embryogenesis from immature inflorescences of oil palm

J. B. Teixeira, M. R. Söndahl, E. G. Kirby

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

Immature inflorescences of oil palm (Elaeis guineensis) var. Pisifera were inoculated onto modified MS medium containing 0.3% (w/v) activated charcoal and 475 μM 2,4-D. After 2-3 months of culture, a hard yellow callus proliferated at the base of the shoot-like structures. The high incidence of phenolic oxidation required the use of increased levels of activated charcoal (0.5% w/v) and 2,4-D (500 μM). Development of floral structures from inflorescence expiants was frequently observed during the culture period. After 81 weeks of culture, an embryogenic tissue characterized by compact consistency and pearly white color was observed in tissues derived from very young inflorescences. This compact embryogenic tissue differentiated into normal somatic embryos when transferred onto regeneration medium containing NAA (15 μM) and ABA (2 μM). Normal plantlets were recovered from these somatic embryos after 8 weeks on regeneration medium.

Original languageEnglish (US)
Pages (from-to)247-250
Number of pages4
JournalPlant Cell Reports
Volume13
Issue number5
DOIs
StatePublished - Feb 1994

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Keywords

  • Inflorescence
  • Oil Palm
  • Somatic Embryogenesis

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