Somatic embryogenesis in cultured mature zygotic embryos of Abies balsamea

Todd G. Guevin, Victor Micah, Edward G. Kirby

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28 Scopus citations


Embryo suspensor masses (ESMs) were induced by culture of isolated mature zygotic embryos of balsam fir [Abies balsamea (Mill.)] on media containing 10 μM cytokinin [6-(γ-γ-dimethylallylamino)purine (2iP), 6-benzyladenine (BA), or thidiazuron (TDZ)]. Once induced, ESMs proliferated on media containing 2iP, BA or TDZ (10 μM) or on 4.5 μM BA in combination with 10 μM naphthyl-1-acetic acid. When ESMs were transferred to media containing 5-80 μM abscisic acid, cotyledonary-stage embryos were formed. Embryos were readily germinated on medium lacking growth regulators.

Original languageEnglish (US)
Pages (from-to)205-208
Number of pages4
JournalPlant Cell, Tissue and Organ Culture
Issue number2
StatePublished - May 1 1994

All Science Journal Classification (ASJC) codes

  • Horticulture


  • conifers
  • cytokinins

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