Some insights on retention and selectivity for hydrophilic interaction chromatography

Mei Yang, Richard Thompson, Gene Hall

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Using a pure silica stationary phase along with a simple acetonitrile-phosphoric acid aqueous mobile phase provides a powerful and versatile tool for the analysis of a broad range of polar compounds, such as amino acids and pharmaceutical compounds. The retention of solutes is influenced by the nature of the stationary phase, the organic solvent, and the aqueous mobile phase components. The retention mechanism is predominantly electrostatic interactions occurring between the solute and stationary phase. Selectivity for cationic or zwitterionic solutes is largely due to differences in their ion exchange capabilities, and, consequently, influenced by the pKa and lipophilicity of the solutes. Steric hindrance in the proximity of the protonated site of the solutes may also influence selectivity and retention.

Original languageEnglish (US)
Pages (from-to)628-646
Number of pages19
JournalJournal of Liquid Chromatography and Related Technologies
Volume32
Issue number5
DOIs
StatePublished - Mar 1 2009

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

Keywords

  • Amino acids
  • Hydrophilic interaction chromatography
  • Polar compounds

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