South (S)- and north (N)-methanocarba-7-deazaadenosine analogues as inhibitors of human adenosine kinase

Kiran S. Toti, Danielle Osborne, Antonella Ciancetta, Detlev Boison, Kenneth A. Jacobson

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Adenosine kinase (AdK) inhibitors raise endogenous adenosine levels, particularly in disease states, and have potential for treatment of seizures, neurodegeneration, and inflammation. On the basis of the South (S) ribose conformation and molecular dynamics (MD) analysis of nucleoside inhibitors bound in AdK X-ray crystallographic structures, (S)- and North (N)-methanocarba (bicyclo[3.1.0]hexane) derivatives of known inhibitors were prepared and compared as human (h) AdK inhibitors. 5′-Hydroxy (34, MRS4202 (S); 55, MRS4380 (N)) and 5′-deoxy 38a (MRS4203 (S)) analogues, containing 7- and N6-NH phenyl groups in 7-deazaadenine, robustly inhibited AdK activity (IC50 ∼ 100 nM), while the 5′-hydroxy derivative 30 lacking the phenyl substituents was weak. Docking in the hAdK X-ray structure and MD simulation suggested a mode of binding similar to 5′-deoxy-5-iodotubercidin and other known inhibitors. Thus, a structure-based design approach for further potency enhancement is possible. The potent AdK inhibitors in this study are ready to be further tested in animal models of epilepsy.

Original languageEnglish (US)
Pages (from-to)6860-6877
Number of pages18
JournalJournal of medicinal chemistry
Volume59
Issue number14
DOIs
StatePublished - Jul 28 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Drug Discovery

Fingerprint

Dive into the research topics of 'South (S)- and north (N)-methanocarba-7-deazaadenosine analogues as inhibitors of human adenosine kinase'. Together they form a unique fingerprint.

Cite this