Invention Summary: Upconversion nanoparticles (UCNPs) have gained increasing attention for various biomedical applications due to their high photostability, low autofluorescent background, and deep tissue penetration; however, UCNPs also suffer from low emission intensities due to undesirable energy back-transfer. Further, the use of high-power density excitations at 980 nm may cause serious overheating and thus cellular damages under continuous irradiation. Researchers at Rutgers have developed a single-crystal core–shell–shell “sandwich” structured UCNP that eliminates energy back-transfer to yield bright visible emissions using low power density excitations. These UCNPs show a remarkable enhancement of luminescent output relative to conventional β-NaYF4:Yb,Er co-doped UCNPs and β-NaYF4:Yb,Er@NaYF4:Yb “active shell” structured UCNPs. As a proof-of-concept, this advanced core–shell–shell UCNP is subsequently used to develop a highly sensitive biosensor for the ultrasensitive detection of dopamine released from stem cell-derived dopaminergic-neurons. Given the challenges of in situ detection of biomolecules, this UCNP-based NIR biosensor presents a unique tool for investigating single-cell mechanisms and understanding their roles in biological processes. Market Applications: Bioimaging Biosensing probe Medical imaging contrast agent Advantages : Low thermal toxicity Efficient excitation-emission conversion at low power density High signal to noise ratio No photobleaching Modular functionalization for diverse biomolecule detection Intellectual Property & Development Status: Patent pending. Patent pending. Available for licensing and/or research collaboration. Publications NIR Biosensing of Neurotransmitters in Stem Cell-Derived Neural Interface Using Advanced Core-Shell Upconversion Nanoparticles.Rabie H, Zhang Y, Pasquale N, Lagos MJ, Batson PE and Lee K-B. Advanced Materials 2019, 31, 1806991.
|Original language||English (US)|
|Publication status||Published - Sep 2019|